Compositions, methods and kits to detect herpes simplex virus nucleic acids
View Patent ↗The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Herpes Simplex Virus (HSV) nucleic acid (e.g., HSV-1 and/or HSV-2 nucleic acid). Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.
1. A Herpes Simplex Virus 1 (HSV-1) detection probe comprising a target-hybridizing sequence and a covalently linked non-nucleotide detectable label, wherein the target hybridizing sequence consists of the sequence of SEQ ID NO: 22.
2. The HSV-1 detection probe of claim 1 , further comprising a quencher.
3. The HSV-1 detection probe of claim 2 , which is a molecular torch.
4. The HSV-1 detection probe of claim 3 , comprising the sequence of SEQ ID NO: 21.
5. A Herpes Simplex Virus 2 (HSV-2) detection probe comprising a target-hybridizing sequence and a covalently linked non-nucleotide detectable label, wherein the target hybridizing sequence consists of the sequence of SEQ ID NO: 27.
6. The HSV-2 detection probe of claim 5 , further comprising a quencher.
7. The HSV-2 detection probe of claim 6 , which is a molecular torch.
8. The HSV-2 detection probe of claim 7 , comprising the sequence of SEQ ID NO: 26.
9. A combination comprising the HSV-1 detection probe of claim 1 and an HSV-2 detection probe comprising a target-hybridizing sequence and a covalently linked non-nucleotide detectable label, wherein the target hybridizing sequence of the HSV-2 detection probe consists of the sequence of SEQ ID NO: 27.
10. The combination of claim 9 , wherein the HSV-1 detection probe further comprises a quencher and the HSV-2 detection probe further comprises a quencher.
11. The combination of claim 10 , wherein the HSV-1 detection probe is a molecular torch and the HSV-2 detection probe is a molecular torch.
12. The combination of claim 11 , wherein the HSV-1 detection probe comprises the sequence of SEQ ID NO: 21 and the HSV-2 detection probe comprises the sequence of SEQ ID NO: 26.
13. A kit comprising the combination of claim 9 .
14. A kit comprising the combination of claim 12 .
15. A method of determining the presence or absence of Herpes Simplex Virus 1 (HSV-1) in a sample, said method comprising contacting a sample suspected of comprising HSV-1 nucleic acid with the HSV-1 detection probe of claim 1 and determining whether the HSV-1 detection probe hybridizes to the HSV-1 nucleic acid.
16. The method of claim 15 , wherein the HSV-1 nucleic acid is an HSV-1 amplification product and the HSV-1 detection probe is configured to specifically hybridize to the HSV-1 amplification product.
17. A method of determining the presence or absence of Herpes Simplex Virus 2 (HSV-2) in a sample, said method comprising contacting a sample suspected of comprising HSV-2 nucleic acid with the HSV-2 detection probe of claim 5 and determining whether the HSV-2 detection probe hybridizes to the HSV-2 nucleic acid.
18. The method of claim 17 , wherein the HSV-2 nucleic acid is an HSV-2 amplification product and the HSV-2 detection probe is configured to specifically hybridize to the HSV-2 amplification product.
19. A method of determining the presence or absence of HSV-1 or HSV-2 in a sample, said method comprising contacting a sample suspected of comprising HSV-1 or HSV-2 nucleic acid with the HSV-1 detection probe of claim 1 and an HSV-2 detection probe comprising a target-hybridizing sequence and a covalently linked non-nucleotide detectable label, wherein the target hybridizing sequence of the HSV-2 detection probe consists of the sequence of SEQ ID NO: 27; and determining whether the HSV-1 detection probe hybridizes to the HSV-1 nucleic acid and whether the HSV-2 detection probe hybridizes to the HSV-2 nucleic acid.
20. The method of claim 19 , wherein the HSV-1 nucleic acid is an HSV-1 amplification product and the HSV-1 detection probe is configured to specifically hybridize to the HSV-1 amplification product; and the HSV-2 nucleic acid is an HSV-2 amplification product and the HSV-2 detection probe is configured to specifically hybridize to the HSV-2 amplification product.