Compositions and methods for the encapsulation and scalable delivery of agrochemicals
The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.
1. An anucleated cell composition comprising: (a) an intact anucleated cell having encapsulated within said cell at least one non-expressed agricultural compound, and wherein said at least one non-expressed agricultural compound is a pesticide.
2. The anucleated cell composition according to claim 1 , further comprising: (b) at least one agriculturally acceptable carrier.
3. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is derived from a prokaryotic cell.
4. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell.
5. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a gram negative bacterial genus.
6. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a bacterial genus selected from the group consisting of: Escherichia, Salmonella, Shigella, Pseudomonas , and Agrobacterium.
7. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a bacterial species selected from the group consisting of: Escherichia coli, Salmonella typhimurium, Shigella flexneri , and Pseudomonas aeruginosa.
8. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a P678-54 E. coli parental bacterial cell.
9. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a gram positive bacterial genus.
10. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a bacterial genus selected from the group consisting of: Bacillus, Corynebacterium , and Lactobacillus.
11. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is produced from a bacterial species selected from the group consisting of: Bacillus subtilis, Corynebacterium glutamicum , and Lactobacillus acidophilus.
12. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a parental bacterial cell deficient in WprA protease.
13. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a protease deficient B. subtilis parental bacterial cell.
14. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a protease deficient KO7 B. subtilis parental bacterial cell.
15. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a protease deficient B. subtilis parental bacterial cell selected from the group consisting of: (1) CU403,DIVIVA; (2) CU403,DIVIVB,SPO-; (3) CU403,DIVIVB; and (4) CU403,DIVIVB1, wherein at least one protease encoding gene has been repressed, deleted, or silenced.
16. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a protease deficient parental bacterial cell.
17. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a parental bacterial cell deficient in Lon and OmpT proteases.
18. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a protease deficient E. coli parental bacterial cell.
19. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is a bacterially derived minicell that is produced from a protease deficient E. coli parental bacterial cell selected from the group consisting of: BL21, BL21(DE3), BL21-AI, LPS-modified BL21 (DE3) and B8.
20. The anucleated cell composition according to claim 1 , wherein said intact anucleated cell is derived from an eukaryotic cell.
21. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is an herbicide.
22. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is a sulfonylurea herbicide.
23. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is foramsulfuron.
24. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is an insecticide.
25. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is a neonicotinoid insecticide.
26. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is clothianidin.
27. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is a keto-enol insecticide.
28. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is spirotetramat.
29. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is a fungicide.
30. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is a strobilurin fungicide.
31. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is pyraclostrobin.
32. The anucleated cell composition according to claim 1 , wherein the at least one non-expressed agricultural compound is a nematicide.
33. The anucleated cell composition according to claim 1 , formulated as a liquid, dry composition, powder, granule, seed coating, drench, in-furrow composition, or foliar spray.
34. A method of delivering at least one non-expressed agricultural compound to a locus, comprising: applying the anucleated cell composition according to claim 1 to a desired locus.
35. A method of delivering at least one non-expressed agricultural compound to a plant, comprising: applying the anucleated cell composition according to claim 1 to a plant.
36. A method of delivering at least one non-expressed agricultural compound to a plant, comprising: applying the anucleated cell composition according to claim 1 to a plant, wherein the plant comprises the seed, stalk, flower, fruit, leaves, roots, or rhizome.
37. A method of delivering at least one non-expressed agricultural compound to a crop, comprising: applying the anucleated cell composition according to claim 1 to a crop or a locus in proximity to said crop.
38. The anucleated cell composition according to claim 1 , wherein the anucleated cell is treated with a solvent.
39. The anucleated cell composition according to claim 38 , wherein said solvent is ethanol, DMSO, polyethylene glycol, or glycerol.
40. The anucleated cell composition according to claim 38 , wherein the anucleated cell is treated with an agent, in addition to said solvent.
41. The anucleated cell composition according to claim 40 , wherein said agent is a fixative, a preservative or a cross-linking agent.
42. The anucleated cell composition according to claim 41 , wherein said cross-linking agent is glutaraldehyde, formaldehyde, genipin, or epigallocatechin gallat.
43. The anucleated cell composition according to claim 38 , wherein said solvent increases solubility of the at least one non-expressed agricultural compound into the anucleated cell.
44. The anucleated cell composition according to claim 38 , wherein said solvent increases solubility of the at least one non-expressed agricultural compound into the anucleated cell, and wherein said solvent increases diffusion of the at least one non-expressed agricultural compound into the anucleated cell.
45. The anucleated cell composition according to claim 40 , wherein said agent captures the at least one non-expressed agricultural compound within a membrane of the anucleated cell.
46. The anucleated cell composition according to claim 40 , wherein said agent captures the at least one non-expressed agricultural compound within a membrane of the anucleated cell, and wherein said agent cross-links the at least one non-expressed agricultural compound to the anucleated cell, which improves stability of the anucleated cell.
47. The anucleated cell composition according to claim 40 , wherein said agent enhances loading capacity of the at least one non-expressed agricultural compound into the anucleated cell.
48. The anucleated cell composition according to claim 40 , wherein said agent enhances loading capacity of the at least one non-expressed agricultural compound into the anucleated cell, and wherein said agent controls a release rate of the at least one non-expressed agricultural compound from the anucleated cell.
49. The anucleated cell composition according to claim 1 , wherein the anucleated cell exhibits a controlled release rate of the at least one non-expressed agricultural compound.
50. The anucleated cell composition according to claim 49 , wherein the anucleated cell exhibits the controlled release rate of the at least one non-expressed agricultural compound, and wherein said at least one non-expressed agricultural compound is released at a steady rate.
51. The anucleated cell composition according to claim 1 , wherein the anucleated cell exhibits an initial burst release of the at least one non-expressed agricultural compound.
52. The anucleated cell composition according to claim 51 , wherein the anucleated cell exhibits the initial burst release of the at least one non-expressed agricultural compound, and wherein said burst release comprises a release of at least about 40% of the at least one non-expressed agricultural compound.
53. The anucleated cell composition according to claim 49 , wherein the anucleated cell exhibits the controlled release rate of the at least one non-expressed agricultural compound, and wherein the controlled release rate is less than 40%, less than 30%, less than 20%, less than 15%, less than 10%, or less than 5% of the at least one non-expressed agricultural compound being released from the anucleated cell per day.
54. The anucleated cell composition according to claim 53 , wherein the controlled release rate is less than 15% of the at least one non-expressed agricultural compound released from the anucleated cell per day.
55. The anucleated cell composition according to claim 53 , wherein the controlled release rate is less than 10% of the at least one non-expressed agricultural compound released from the anucleated cell per day.
56. The anucleated cell composition according to claim 53 , wherein the controlled release rate is about 10% of the at least one non-expressed agricultural compound released from the anucleated cell per day.