METHODS AND COMPOSITIONS FOR TREATING NEUROLOGICAL DISORDERS
Disclosed herein are methods and compositions for cell induction and treating neurological disorders, utilizing adherent stromal cells, which may, for example, be derived from placental tissue, bone marrow, or adipose tissue. Also provided are pharmaceutical compositions comprising the described cells, optionally in combination with pharmaceutically acceptable excipients.
1 . A method of treating a neurodegenerative disease in a subject in need thereof, comprising the step of administering to said subject a pharmaceutical composition comprising induced adherent stromal cells (ASC), thereby treating a neurodegenerative disease.
2 . The method of claim 1 , wherein said neurodegenerative disease is Alzheimer's disease.
3 . The method of claim 1 , wherein said neurodegenerative disease is Parkinson's disease.
4 . The method of claim 1 , wherein said neurodegenerative disease is Amyotrophic lateral sclerosis (ALS).
5 . The method of claim 1 , wherein said neurodegenerative disease is Huntington's disease.
6 . The method of claim 1 , wherein said neurodegenerative disease is multiple sclerosis (MS), spinal muscular atrophy, spinal cord injury, spinocerebellar ataxia, or an autism spectrum disorder.
7 - 10 . (canceled)
11 . The method of claim 1 , wherein said administering is selected from intranasal administration, intracerebral administration, intracerebroventricular administration, intrathecal administration, intravenous administration, and intramuscular administration.
12 . The method of claim 1 , wherein said ASC have been induced by incubation in an induction medium comprising heparin and cyclic AMP (cAMP) or an analogue thereof.
13 . The method of claim 12 , wherein said induction medium further comprises an induction agent selected from basic fibroblast growth factor (b-FGF), PDGF (platelet-derived growth factor), and Neuregulin.
14 - 17 . (canceled)
18 . The method of claim 12 , wherein said induction medium further comprises serum.
19 . The method of claim 12 , wherein said induction medium is serum free.
20 . The method of claim 12 , wherein said ASC were expanded ex vivo prior to inducing said ASC.
21 . The method of claim 20 , wherein said ASC are expanded on a 2D substrate, and then induced on a 3D substrate.
22 . The method of claim 12 , wherein said ASC have been incubated in a serum-free medium, prior to incubation in said induction medium.
23 . The method of claim 1 , wherein said ASC have been induced by incubation in a serum-free medium comprising PDGF, bFGF, and TGF β.
24 . (canceled)
25 . The method of claim 23 , wherein said medium further comprises cAMP.
26 . The method of claim 23 , wherein said ASC have been incubated on a 3D substrate, following said incubation in a serum-free medium, wherein said 3D substrate culture apparatus comprises a synthetic adherent material.
27 - 31 . (canceled)
32 . A method of inducing ASC to secrete a neurotrophic or neuroprotective growth factor, comprising incubating said ASC in an induction medium comprising heparin and cAMP or a cAMP analogue.
33 . The method of claim 32 , wherein said induction medium further comprises an induction agent selected from basic fibroblast growth factor (b-FGF), PDGF (platelet-derived growth factor), and Neuregulin.
34 - 45 . (canceled)
46 . The method of claim 1 , wherein said ASC originate from placental tissue.
47 - 50 . (canceled)