Macrocyclic polypeptides
Disclosed herein are macrocyclic polypeptides having no more than 3 ammo acid substitutions compared to the amino acid sequence of any one of SEQ ID NO: 1-2.37 or a mirror image thereof, wherein the polypeptide includes both L and D amino acids, libraries of such polypeptides, and uses thereof.
1. A macrocyclic polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof, wherein the polypeptide is N-terminus to C-terminus cyclized.
2. The macrocyclic polypeptide of claim 1 , wherein the macrocyclic polypeptide is between 7 and 14 amino acid residues in length, or between 7 and 10 amino acid residues in length.
3. A polypeptide library, comprising two or more different polypeptides comprising the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof, wherein the polypeptides are N-terminus to C-terminus cyclized.
4. The polypeptide library of claim 3 , comprising ten or more different polypeptides comprising the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof.
5. The polypeptide library of claim 4 , comprising fifty or more different polypeptides comprising the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof.
6. The polypeptide library of claim 5 , comprising two hundred or more different polypeptides comprising the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof.
7. The macrocyclic polypeptide of claim 1 , consisting of the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof.
8. The polypeptide library of claim 3 , comprising two or more polypeptides consisting of the amino acid sequence of any one of SEQ ID NO: 1-237 or a mirror image thereof, wherein the polypeptides are N-terminus to C-terminus cyclized.
9. The macrocyclic polypeptide of claim 1 , wherein the macrocyclic polypeptide comprises the amino acid sequence of SEQ ID NO:234.
10. The macrocyclic polypeptide of claim 1 , wherein the macrocyclic polypeptide consists of the amino acid sequence of SEQ ID NO:234.