Mechanical method of maintaining narrow residence time distributions in continuous flow systems
Methods of maintaining narrow residence time distributions in continuous flow systems, particularly applicable to virus inactivation such as during a protein purification process. Fluid sample is introduced into an axial flow channel and caused to flow therein in discrete packets or zones to minimize residence time distribution and axial dispersion. Embodiments described herein obviate or minimize the need for using large tanks or reservoirs for performing virus inactivation during a protein purification process; reduce the overall time required for virus inactivation, and/or reduce the overall physical space required to perform the virus inactivation operation during a protein purification process, which in turn reduces the overall footprint for the purification process.
1. A method for inactivating one or more viruses in a sample containing a target molecule, wherein the method comprises continuously exposing said sample to virus inactivation conditions, followed by causing the sample to flow in a flow channel in discrete packets by applying compressive force to the flow channel during a process for purifying said target molecule, wherein said compressive force is caused by compressing tubing coiled around a mandrel, said tubing defining said flow channel.
2. The method of claim 1 , wherein said flow channel has an outer wall, and wherein said compressive force is created by pinching said outer wall.
3. The method of claim 2 , comprising pinching said outer wall with one or more rollers.