Transmembrane pore consisting of two CsgG pores
Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.
1. A method of characterising a polynucleotide using a transmembrane pore, the method comprising translocating the polynucleotide through the transmembrane pore, wherein the transmembrane pore comprises a first CsgG pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof, arranged vertically to form a channel through a membrane where the tail of the first CsgG pore associates with the tail of the second CsgG pore.
2. A method according to claim 1 , wherein the polynucleotide comprises a homopolymeric region.
3. A method according to claim 1 , wherein the first CsgG pore, or homologue thereof, is a homooligomer and the second CsgG pore, or homologue thereof, is a homooligomer.
4. The method of claim 3 , wherein the first CsgG pore, or homologue thereof, comprises monomers that have a different amino acid sequence from the monomers of which the second CsgG pore, or homologue thereof, is comprised.
5. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a homooligomer and the second CsgG pore, or homologue thereof, is a homooligomer, and the first CsgG pore, or homologue thereof, and/or the second CsgG pore, or homologue thereof is not a wild-type CsgG pore.
6. The method of claim 5 , wherein the first CsgG pore or homologue thereof, and the second CsgG pore, or homologue thereof comprise identical amino acid sequences.
7. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a heterooligomer and the second CsgG pore, or homologue thereof, is a homooligomer.
8. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a homooligomer and the second CsgG pore, or homologue thereof, is a heterooligomer.
9. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a heterooligomer and the second CsgG pore, or homologue thereof, is a heterooligomer.
10. The method of claim 9 , wherein the first CsgG pore, or homologue thereof, and the second CsgG pore, or homologue thereof comprise identical amino acid sequences.