Membrane-impermeant fluorogenic chromophores
Fluorescent labeling of proteins. In particular, membrane-impermeant fluorogenic chromophores being capable of binding reversibly a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labeling biological molecules of interest, preferably proteins of interest. Especially, 4-hydroxybenzylidene-rhodanine (HBR) analogs of formula (II) as membrane-impermeant fluorogenic chromophores.
1. A complex formed by a compound of formula (III):
or a salt thereof;
with a photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof.
2. The complex according to claim 1 , wherein the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof, binds the compound of formula (III) with a K D lower than about 15 μM when measured at a temperature of about 25° C.
3. A method for detecting a tagged biological molecule of interest in a sample comprising compartments enclosed by at least one membrane, comprising the steps of:
fusing a photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof, to a biological molecule of interest, thereby tagging the biological molecule of interest with the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof to provide the tagged biological molecule of interest; contacting the sample with a membrane-impermeant fluorogenic chromophore of formula (III):
or a salt thereof; and
detecting a fluorescence resulting from the binding of the membrane-impermeant fluorogenic chromophore of formula (III) to the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof; thereby detecting the tagged biological molecule of interest present at least in part at the extra membranous surface of the compartments, or secreted from the compartments, through the binding of the membrane-impermeant fluorogenic chromophore of formula (III) to the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof.
4. The method according to claim 3 , wherein the tagged biological molecule of interest is a membrane protein with at least a part of said protein extruding on the outside of said membrane or a secreted protein.
5. The method according to claim 3 , wherein the binding of the membrane-impermeant fluorogenic chromophore of formula (III) to the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof, is reversible.
6. The method according to claim 3 , further comprising a step of quantifying the tagged biological molecule of interest by measuring the fluorescence emitted upon binding of the membrane-impermeant fluorogenic chromophore of formula (III) to the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof, tagged to the biological molecule of interest.
7. The method according to claim 3 , wherein the tagged biological molecule of interest is a protein of interest and wherein the method is for sequentially labeling said protein of interest, said method comprising:
fusing the photoactive yellow protein (PYP) functional derivative, or a functional fragment thereof, to the protein of interest, thereby tagging the protein of interest with the PYP functional derivative, or a functional fragment thereof, to provide the tagged protein of interest;
contacting the sample comprising the tagged protein of interest with the membrane-impermeant fluorogenic chromophore of formula (III);
detecting a fluorescence resulting from the binding of the membrane-impermeant fluorogenic chromophore of formula (III) to the PYP functional derivative, or a functional fragment thereof, thereby detecting the fraction of the tagged protein of interest present at least in part at the extra membranous surface of the compartment, or secreted from said compartment;
contacting the sample comprising the tagged protein of interest with a membrane-permeant fluorogenic chromophore able to specifically bind to the tagged protein of interest; detecting a fluorescence resulting from the binding of the membrane-permeant fluorogenic chromophore to the tagged protein of interest, thereby detecting the whole population of the tagged protein of interest.
8. The method according to claim 3 , wherein the biological molecule of interest is a reporter protein and wherein the method is for assessing the activity of another protein of interest involved in the expression or the anchoring of the reporter protein at the membrane or in the secretion of the reporter protein through the detection of the reporter protein in the sample comprising compartments enclosed by at least one membrane.
9. A compound of formula (III):
or a salt thereof.