Methods and pharmaceutical compositions for the treatment of mast cell diseases
The inventors demonstrate for the first time the activation of the Hedgehog (HH) signaling pathway in normal and abnormal human mast cells (MCs). These results prompt the inventors to explore the consequence of the inhibition of the HH pathway, especially the canonical pathway, on MC proliferation. They demonstrate that Hedgehog inhibitors inhibit proliferation and induces apoptosis of mast cells. Accordingly the present invention relates to a method of treating a mast cell disease in a patient in need there of comprising administering to the patient a therapeutically effective amount of a Hedgehog inhibitor.
1. A method of treating mastocytosis in a patient in need thereof comprising administering to the patient a therapeutically effective amount of a Hedgehog inhibitor, wherein the therapeutically effective amount is an amount sufficient to inhibit mast cell proliferation.
2. The method of claim 1 wherein the mastocytosis is selected from the group consisting of cutaneous mastocytosis; systemic mastocytosis; and systemic indolent mastocytosis.
3. The method of claim 1 wherein the pathogenesis of mastocytosis is attributed to constitutive activation of the receptor tyrosine kinase KIT.
4. The method of claim 1 wherein the Hedgehog inhibitor is vismodegib or GANT61.
5. The method of claim 1 wherein the Hedgehog inhibitor is administered to the patient in combination with a tyrosine kinase inhibitor selected from the group consisting of PKC412 (midostaurin), afatinib, alitretinoin, axitinib, bafetinib, bexarotene, BI-2536, bosutinib, brivanib, canertinib, cediranib, CP724714, crizotinib, dasatinib, danusertib, dovitinib, E7080, erlotinib, everolimus, fostamatinib, gefitinib, imatinib, lapatinib, lestaurtinib, linsitinib, masitinib, motesanib, neratinib, nilotinib, NVP TAE-684, OSI-027, OSI-420, OSI-930, pazopanib, pelitinib, PF573228, regorafenib, romidepsin, ruxolitinib, saracatinib, sorafenib, sunitinib, TAE226, TAE684, tandutinib, telatinib, tautinib, temsirolimus, toceranib, tofacitinib, tozasertib, tretinoin, vandetanib, vatalanib, vemurafenib, vorinostat and WZ 4002.
6. The method of claim 3 , wherein the constitutive activation of the receptor tyrosine kinase KIT is due to a KIT mutation.
7. The method of claim 1 , wherein the patient does not have cancer.