Compositions and methods for obtaining organoids
A method for obtaining epithelial organoids is provided. In one embodiment, the method comprises culturing one or more epithelial ducts, epithelial duct fragments and/or epithelial stem cells isolated therefrom in contact with an extracellular matrix in the presence of a basal medium, wherein the medium is free of FGF and/or nicotinamide. Organoids obtained by the methods described herein, and uses thereof, are also provided.
1. A medium for obtaining an epithelial organoid, the medium comprising a basal medium and a Wnt agonist, wherein the medium is (a) free from FGF and/or nicotinamide and (b) free from an inhibitor of BMP signalling and wherein the medium is capable of supporting the organoids for 5 or more passages.
2. The medium of claim 1 , wherein the Wnt agonist is selected from one or more of CHIR99021, Wnt, Wnt3a, Norrin, R-Spondin 1, R-Spondin 2, R-Spondin 3, R-Spondin 4 and a GSK inhibitor.
3. The medium of claim 1 , further comprising one or more of a B27 component, a N2 component and/or N-Acetylcysteine.
4. The medium of claim 1 , further comprising an extracellular matrix, optionally wherein the extracellular matrix concentration ranges between 0.1 to 50% (v/v).
5. The medium of claim 4 , wherein a concentration of the extracellular matrix is about 0.1% (v/v) or less.
6. A medium for obtaining an epithelial organoid, the medium comprising a basal medium and one or more of a B27 component, a N2 component, and/or N-Acetylcysteine, wherein the medium is:
i) free of FGF and/or nicotinamide, free of an activator of the Wnt-beta-catenin pathway, free of a member of the EGF family and free of an inhibitor of BMP signaling, or
ii) free of FGF and/or nicotinamide, free of a member of the EGF family, free of an inhibitor of BMP signaling, and supplemented with one or more activators of the Wnt-beta-catenin pathway, or
iii) free of FGF and/or nicotinamide, free of an inhibitor of BMP signaling, and supplemented with one or more activators of the Wnt-beta-catenin pathway and one or more members of the EGF family, and
wherein the medium supports the organoids for 5 or more passages.
7. The medium of claim 1 , further comprising one or more members of the EGF family.
8. The medium of claim 7 , wherein the one or more members of the EGF family is EGF.