Genetically engineered land plants that express a plant CCP1-like mitochondrial transporter protein
A genetically engineered land plant that expresses a plant CCP1-like mitochondrial transporter protein is provided. The genetically engineered land plant comprises a modified gene for the plant CCP1-like mitochondrial transporter protein. The plant CCP1-like mitochondrial transporter protein is an ortholog of CCP1 of Chlamydomonas reinhardtii of SEQ ID NO: 1 derived from a source land plant. The plant CCP1-like mitochondrial transporter protein is localized to mitochondria of the genetically engineered land plant based on a mitochondrial targeting signal intrinsic to the plant CCP1-like mitochondrial transporter protein. The modified gene comprises (i) a promoter and (ii) a nucleic acid sequence encoding the plant CCP1-like mitochondrial transporter protein. The promoter is non-cognate with respect to the nucleic acid sequence. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the plant CCP1-like mitochondrial transporter protein.
1. A genetically engineered land plant that expresses a plant protein of Zea nicaraguensis of SEQ ID NO: 7, the genetically engineered land plant comprising a modified gene for the plant protein, wherein:
the modified gene comprises (i) a promoter and (ii) a nucleic acid sequence encoding the plant protein;
the promoter is non-cognate with respect to the nucleic acid sequence;
the modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the plant protein; and
the genetically engineered land plant is an oilseed plant.
2. The genetically engineered land plant of claim 1 , wherein the promoter is a constitutive promoter.
3. The genetically engineered land plant of claim 1 , wherein the promoter is a seed-specific promoter.
4. The genetically engineered land plant of claim 1 , wherein the modified gene is integrated into genomic DNA of the genetically engineered land plant.
5. The genetically engineered land plant of claim 1 , wherein the modified gene is stably expressed in the genetically engineered land plant.
6. The genetically engineered land plant of claim 1 , wherein the genetically engineered land plant is an oilseed crop plant selected from the group consisting of camelina, Brassica species, Brassica napus, Brassica rapa, Brassica juncea, Brassica carinata, crambe , soybean, sunflower, safflower, oil palm, flax, and cotton.