Method for Compiling A Genomic Database for A Complex Disease And Method for Using The Compiled Database to Identify Genetic Patterns in The Complex Disease to Establish Diagnostic Biomarkers

1 . A method for preparing a genomic database for a complex disease, the method comprising:

selecting a complex disease;

recruiting patients diagnosed with the selected complex disease using online platforms;

requesting donation of genomic data from the recruited patients;

collecting and de-identifying donated genomic data from the recruited patients; and

storing collected genomic data on a computer-readable medium, thereby preparing the genomic database for the complex disease.

2 . The method of claim 1 , wherein the complex disease is Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).

3 . The method of claim 1 , further comprising:

using at least one computer to prepare a questionnaire including questions about the selected complex disease;

requesting completion of the questionnaire by the recruited patients;

collecting completed questionnaires from the recruited patients; and

storing the completed questionnaires on a computer-readable medium.

4 . The method of claim 3 , wherein the questionnaire includes questions regarding disease symptoms and disease severity.

5 . The method of claim 4 , wherein the complex disease is Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).

6 . A genomic database for a complex disease prepared according to the method of claim 1 .

7 . A genomic database for a complex disease prepared according to the method of claim 4 .

8 . A method for identifying relevant frequencies of single nucleotide polymorphisms (SNPs) associated with a complex disease in a disease population, the method comprising:

preparing a genomic database according to claim 1 ;

identifying single nucleotide polymorphisms (SNPs) in the genomic database;

calculating the relevant frequencies of the identified SNPs; and

comparing frequencies of the identified SNPs to a healthy population to determine association with the complex disease.

9 . The method of claim 8 , wherein the complex disease is Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).

10 . The method of claim 9 , wherein the identified single nucleotide polymorphisms (SNPs) are located on chromosome 11 of the methylenetetrahydrofolate reductase (MTHFR) gene.

11 . A method for determining susceptibility to Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) in a subject, the method comprising:

determining genotype of the subject at chromosome 11; and

identifying a single nucleotide polymorphism (SNP) at any of positions chromosome 11, 800, 251; chromosome 11, 801, 166; chromosome 11, 794, 766; and chromosome 11, 795, 161,

wherein identification of a SNP indicates susceptibility to ME/CFS in the subject.

12 . A method for prophylactically treating a subject for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS), the method comprising:

determining susceptibility to Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) in the subject with the method of claim 11 ; and

if the identification of a SNP indicates susceptibility to ME/CFS in the subject, then treating the subject for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).

13 . The method of claim 12 , wherein treating the subject includes at least one of establishing a daily exercise routine, managing diet and nutrition, administering vitamins and supplements, and administering prescription medication.

14 . The method of claim 13 , wherein treating includes administering prescription medication.