Maize RBCS7A promoter for expressing a ferredoxin-thioredoxin reductase in a plant
Compositions and methods for improving plant growth are provided herein. The maize RbcS7A promoter is provided which is a developmentally regulated promoter. Polynucleotides encoding ferredoxin-thioredoxin reductase proteins, polypeptides encompassing ferredoxin-thioredoxin reductase proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
1. A method for increasing crop yield comprising transforming a plant with at least one ferredoxin-thioredoxin reductase protein-encoding sequence, wherein said ferredoxin-thioredoxin reductase protein-encoding sequence encodes a protein comprising an amino acid sequence having at least 90% sequence identity to a selected from the group consisting of SEQ ID NOs:2 and 14-106, wherein said protein has ferredoxin-thioredoxin reductase activity; and
wherein said ferredoxin-thioredoxin reductase protein-encoding sequence is operably linked to a promoter comprising the nucleic acid sequence set forth SEQ ID NO: 5.
2. The method of claim 1 , wherein said ferredoxin-thioredoxin reductase protein-encoding sequence encodes a protein selected from the group consisting of SEQ ID NOs:2 and 14-106.
3. A plant having stably incorporated into its genome a promoter that drives expression in a plant operably linked to a ferredoxin-thioredoxin reductase protein-encoding sequence, wherein said promoter is heterologous to said ferredoxin-thioredoxin reductase protein-encoding sequence, wherein said ferredoxin-thioredoxin reductase protein-encoding sequence encodes a protein comprising an amino acid sequence having at least 90% sequence identity to a selected from the group consisting of SEQ ID NOs:2 and 14-106, wherein said protein has ferredoxin-thioredoxin reductase activity; and
wherein said promoter comprises the nucleic acid sequence set forth SEQ ID NO: 5.
4. The plant of claim 3 , wherein said ferredoxin-thioredoxin reductase protein-encoding sequence encodes a protein selected from the group consisting of SEQ ID NOs:2 and 14-106.
5. Transformed seed of the plant of claim 3 , wherein said transformed seed comprises said ferredoxin-thioredoxin reductase protein-encoding sequence operably linked to said promoter.
6. The plant of claim 3 wherein said plant is a monocot.
7. The plant of claim 3 wherein said plant is a dicot.
8. A DNA construct comprising, in operable linkage,
a. A promoter that is functional in a plant cell, wherein said promoter comprises the nucleic acid sequence set forth SEQ ID NO: 5; and,
b. A nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein, wherein said nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein encodes a protein comprising an amino acid sequence having at least 90% sequence identity to a selected from the group consisting of SEQ ID NOs:2 and 14-106, wherein said protein has ferredoxin-thioredoxin reductase activity.
9. The DNA construct of claim 8 , wherein said nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein comprises SEQ ID NO:1, or encodes a protein selected from the group consisting of SEQ ID NOs:2 and 14-106.
10. The method of claim 1 , wherein said ferredoxin-thioredoxin reductase protein-encoding sequence comprises a nucleic acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:1, and encodes a protein having ferredoxin-thioredoxin reductase activity.
11. The method of claim 1 , wherein said ferredoxin-thioredoxin reductase protein-encoding sequence comprises SEQ ID NO:1.
12. The plant of claim 3 , wherein said ferredoxin-thioredoxin reductase protein-encoding sequence comprises a nucleic acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:1, and encodes a protein having ferredoxin-thioredoxin reductase activity.
13. The plant of claim 3 , wherein said ferredoxin-thioredoxin reductase protein-encoding sequence comprises SEQ ID NO:1.
14. The DNA construct of claim 8 , wherein said nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein comprises a nucleic acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:1, and encodes a protein having ferredoxin-thioredoxin reductase activity.
15. The DNA construct of claim 8 , wherein said nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein comprises SEQ ID NO:1.