METHODS AND COMPOSITIONS FOR TREATING ADDICTIONS
Disclosed herein are methods and compositions comprising adherent stromal cells.
1 . A method of treating an addiction in a subject in need thereof, comprising: administering to the subject a pharmaceutical composition, comprising placental adherent stromal cells (ASC), thereby treating an addiction.
2 . A method of treating an addiction in a subject in need thereof, comprising: administering to the subject a pharmaceutical composition, comprising adherent stromal cells (ASC) not derived from placental tissue, thereby treating an addiction.
3 . The method of claim 1 , wherein said administering comprises:
a. administering to the subject a first pharmaceutical composition, comprising ASC from a first donor; and
b. administering to said subject, at least 7 days after step a), a second pharmaceutical composition comprising allogeneic ASC from a second donor, wherein said second donor differs from said first donor in at least one allele group of human leukocyte antigen (HLA)-A or human leukocyte antigen (HLA)-B,
4 . The method of claim 3 , further comprising administering to said subject, at least 7 days after step b), a third pharmaceutical composition comprising allogeneic ASC of a third donor, wherein said third donor differs from both said first donor and said second donor in at least one allele group of HLA-A or HLA-B.
5 . The method of claim 1 , wherein said ASC secrete a factor selected from BDNF (brain derived neurotrophic factor), GDNF (glial cell line-derived neurotrophic factor), bFGF (basic fibroblast growth factor), NGF (beta-nerve growth factor), VEGF (vascular endothelial growth factor), and HGF (hepatocyte growth factor), and LIF (Leukemia inhibitory factor).
6 . The method of claim 1 , wherein said ASC have been incubated in a 3D culture apparatus.
7 . The method of claim 6 , further comprising the subsequent step of harvesting said ASC by removing said ASC from said 3D culture apparatus.
8 . The method of claim 6 , wherein said ASC have been incubated in a 2D adherent-cell culture apparatus, prior to said incubation in a 3D culture apparatus.
9 . The method of claim 1 , wherein said 3D culture apparatus comprises a bioreactor.
10 - 12 . (canceled)
13 . The method of claim 1 , wherein said 3D culture apparatus comprises microcarriers.
14 . (canceled)
15 . The method of claim 1 , wherein said addiction is an addiction to a psychostimulant.
16 - 18 . (canceled)
19 . The method of claim 1 , wherein said ASC express a marker selected from the group consisting of CD73, CD90, CD29 and CD105.
20 . The method of claim 19 , wherein said ASC do not express a marker selected from the group consisting of CD3, CD4, CD11b, CD14, CD19, and CD34.
21 . The method of claim 19 , wherein said ASC do not express a marker selected from the group consisting of CD3, CD4, CD34, CD39, and CD106.
22 . The method of claim 21 , wherein less than 50% of said ASC express CD200.
23 . The method of claim 21 , wherein more than 50% of said ASC express CD200.
24 - 26 . (canceled)
27 . The method of claim 2 , wherein said ASC originate from adipose tissue.
28 . The method of claim 2 , wherein said ASC originate from bone marrow.
29 - 31 . (canceled)
32 . The method of claim 1 , wherein the cells are administered intranasally.
33 . The method of claim 1 , wherein the cells are administered intra-cerebro-ventricularly.