Systems and methods for the control of acute hepatopancreatic necrosis disease
Generally, the inventive technology relates to novel strategies for disease control in animal systems. Specifically, the inventive technology relates to novel methods, systems and compositions for the biocontrol of pathogens in aquatic systems. Specifically, the invention may comprise novel techniques, systems, and methods for the biocontrol of disease-transmitting pathogens affecting shrimp in aquaculture systems.
1. A compound for the biocontrol of pathogenic bacteria comprising:
a treated feed, a solid inoculum, or a liquid inoculum for a target host infected with or susceptible to a pathogenic bacteria, said treated feed, solid inoculum, or liquid inoculum comprising a genetically modified donor bacteria that may be introduced to a target host or said target host's environment, wherein said genetically modified donor bacteria is configured to express heterologous lsr operon or aidH and wherein:
said heterologous nucleic acid comprises a nucleotide sequence according to SEQ ID NO:1 or a sequence having approximately 75% or more homology with SEQ ID NO:1 which encodes for said heterologous aidH as a quorum quenching molecule configured to remove exogenous autoinducer-1 (AI-1) molecules from the environment; or
said heterologous nucleic acid comprises a nucleotide sequence according to SEQ ID NO:2 or a sequence having approximately 75% or more homology with SEQ ID NO:2 which encodes for said heterologous lsr operon as a quorum quenching molecule configured to remove exogenous autoinducer-2 (AI-2) molecules from the environment resulting in the reduction or inhibition of quorum sensing (QS) in said pathogenic bacteria.
2. The compound of claim 1 , wherein said target host comprises an aquatic organism.
3. The compound of claim 2 , wherein said aquatic organism comprises a shrimp.
4. The compound of claim 3 , wherein said target host's environment comprises an aquaculture environment.
5. The compound of claim 4 , wherein said pathogenic bacteria comprises an Early Mortality Syndrome (EMS) causing pathogenic bacteria.
6. The compound of claim 5 , wherein said Early Mortality Syndrome (EMS) causing pathogenic bacteria comprises a species of Vibrio.
7. The compound of claim 6 , wherein said lsr operon is operably linked to a promotor that is configured to express at least one ATP-binding cassette transporter (ABC transporter) and at least one chaperone protein that actively pump exogenous autoinducer molecule AI-2 from the environment into said genetically modified bacterial cell.
8. The compound of claim 7 , wherein said promotor comprises a constitutive or an inducible promotor.
9. The compound of claim 1 , wherein said genetically modified bacteria comprises a genetically modified bacteria selected from the group consisting of: an enteric genetically modified donor bacteria, a symbiotic genetically modified donor bacteria, a shrimp probiotic genetically modified donor bacteria, an shrimp symbiotic genetically modified donor bacteria, an shrimp endosymbiotic genetically modified donor bacteria, an shrimp enteric genetically modified donor bacteria, Ag1 , Salmonella typhimurium, Bacillus subtilis, Enterobacter , and E. coli , or other symbiotic bacteria.
10. The compound of claim 1 , wherein said genetically modified bacteria configured to express said heterologous lsr operon may be transmitted through vertical or horizontal transfer.
11. The compound of claim 1 , wherein said genetically modified bacteria configured to express a said heterologous lsr operon comprises a genetically modified bacteria configured to express a said heterologous lsr operon wherein said levels of exogenous AI-2 molecules in the environment are reduced to a level where biofilm formation or expression of pathogenesis genes, including those involved in toxin production by said pathogenic bacteria is reduced, or inhibited.
12. The compound of claim 1 , wherein said genetically modified bacteria configured to express a said heterologous lsr operon comprises a genetically modified bacteria configured to express a said heterologous lsr operon wherein said levels of exogenous AI-2 molecules in the environment are reduced to a level where QS-mediated pathogenicity of said pathogenic bacteria is reduced, or inhibited.
13. The compound of claim 1 wherein said genetically modified bacteria configured to express a said heterologous lsr operon comprises a genetically modified bacteria configured to express a said heterologous lsr operon wherein said levels of exogenous AI-2 molecules in the environment are reduced to a level where EMS caused by Vibrio is treated or prevented.
14. The compound of claim 1 wherein said genetically modified bacteria configured to express a said heterologous lsr operon comprises a genetically modified bacteria configured to express said heterologous lsr operon wherein said levels of exogenous AI-2 molecules in the environment are reduced to a level where mortality of said target host due to EMS caused by one or more Vibrio is reduced.
15. A method of treating Early Mortality Syndrome (EMS) in an organism comprising introducing a compound according to claim 1 to a target host or said target host's environment that is infected by, or susceptible to infection by, an EMS-causing bacterial pathogen, wherein said target host is a shrimp, wherein said target host's environment comprises an aquaculture environment, and wherein said EMS-causing pathogen comprises a species of Vibrio.