Chromatography carrier, ligand-immobilizing carrier, chromatography column, target substance purification method, and chromatography carrier production method
To provide a chromatography carrier that has excellent antifouling properties and exhibits excellent pressure-resistant performance and shatter-resistant performance. A chromatography carrier comprising: a polymer having a partial structure containing at least two groups represented by —C(═O)—NH—.
1. A ligand-immobilizing carrier, comprising:
a chromatography carrier; and
an antibody affinity ligand immobilized to the chromatography carrier,
wherein the chromatography carrier comprises a polymer having a structural unit (A) of formula (9) and a structural unit (B) of formula (10), and wherein a residue of a functional group capable of immobilizing a ligand in the structural unit (B) is crosslinked by a crosslinked structure of formula (2-1) or (2-4), the formula (9) is
where R 8 is a hydrogen atom or a methyl group, R 9 is a single bond or a divalent linking group, and Z 1 is a functional group capable of immobilizing a ligand, and the formula (10) is
where R 10 is a hydrogen atom or a methyl group, R 11 is a single bond or a divalent linking group, Z 2 is a residue of a functional group capable of immobilizing a ligand, and ** is a bond that is bonded to the crosslinked structure of the formula (2-1) or (2-4)
where R 2 is a single bond or a divalent linking group, * is a chemical bond, and each of R 4a and R 4b is independently a divalent linking group.
2. The ligand-immobilizing carrier of claim 1 , wherein the crosslinked structure has the formula (2-1).
3. The ligand-immobilizing carrier of claim 1 , wherein the polymer further comprises a structural unit derived from an aromatic vinyl-based crosslinkable monomer.
4. The ligand-immobilizing carrier of claim 1 , wherein the antibody affinity ligand is an immunoglobulin-binding protein.
5. The ligand-immobilizing carrier of claim 1 , wherein R 2 is (i) a divalent hydrocarbon group having 1 to 40 carbon atoms or (ii) a group comprising an ether bond, an amide bond, an ester bond, or two or more of any of these, between carbon-carbon atoms of a divalent hydrocarbon group having 2 to 40 carbon atoms.
6. The ligand-immobilizing carrier of claim 1 , wherein R 2 is (i) an alkanediyl group having 1 to 40 carbon atoms or (ii) a group comprising an ether bond, an amide bond, an ester bond, or two or more of any of these, between carbon-carbon atoms of an alkanediyl group having 2 to 40 carbon atoms.
7. The ligand-immobilizing carrier of claim 1 , wherein the antibody affinity ligand is protein A, protein G, or protein L.
8. The ligand-immobilizing carrier of claim 1 , wherein the chromatography carrier is a particulate chromatography carrier.
9. The ligand-immobilizing carrier of claim 1 , wherein the chromatography carrier is a porous chromatography carrier.
10. The ligand-immobilizing carrier of claim 2 , wherein the polymer further comprises a structural unit derived from an aromatic vinyl-based crosslinkable monomer.
11. The ligand-immobilizing carrier of claim 2 , wherein the antibody affinity ligand is an immunoglobulin-binding protein.
12. The ligand-immobilizing carrier of claim 2 , wherein R 2 is (i) a divalent hydrocarbon group having 1 to 40 carbon atoms or (ii) a group comprising an ether bond, an amide bond, an ester bond, or two or more of any of these, between carbon-carbon atoms of a divalent hydrocarbon group having 2 to 40 carbon atoms.
13. The ligand-immobilizing carrier of claim 2 , wherein R 2 is (i) an alkanediyl group having 1 to 40 carbon atoms or (ii) a group comprising an ether bond, an amide bond, an ester bond, or two or more of any of these, between carbon-carbon atoms of an alkanediyl group having 2 to 40 carbon atoms.
14. The ligand-immobilizing carrier of claim 2 , wherein the antibody affinity ligand is protein A, protein G, or protein L.
15. The ligand-immobilizing carrier of claim 2 , wherein the chromatography carrier is a particulate chromatography carrier.
16. The ligand-immobilizing carrier of claim 2 , wherein the chromatography carrier is a porous chromatography carrier.
17. A chromatography column, comprising:
the ligand-immobilizing carrier recited in claim 1 ; and
a column container,
wherein the carrier is comprised in the column container.
18. A method for purifying a target substance by chromatography, comprising:
bringing the ligand-immobilizing carrier recited in claim 1 into contact with the target substance.
19. A method for purifying a target substance by chromatography, comprising:
bringing the ligand-immobilizing carrier recited in claim 1 into contact with a sample containing the target substance.