Increasing plant growth and yield by using a glutaredoxin
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding glutaredoxin proteins, polypeptides encompassing glutaredoxin proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
1. A method for increasing crop yield comprising transforming a plant with at least one glutaredoxin protein-encoding sequence;
wherein the glutaredoxin protein-encoding sequence encodes a protein which shares at least 80% sequence identity with SEQ ID NO 3;
wherein the glutaredoxin protein-encoding sequence is operably linked to a heterologous bundle sheath cell-preferred promoter which comprises SEQ ID NO 10.
2. The method of claim 1 , wherein said glutaredoxin protein-encoding sequence comprises a sequence selected from the group of SEQ ID NOs:1 and 2, or encodes a protein selected from the group consisting of SEQ ID NOs:3-, 15-17, 18, 20, 21, 24-26 and 29.
3. A plant having stably incorporated into its genome a promoter that drives expression in a plant operably linked to a glutaredoxin protein-encoding sequence, wherein said promoter is heterologous to said glutaredoxin protein-encoding sequence;
wherein the glutaredoxin protein-encoding sequence encodes a protein which shares at least 80% sequence identity with SEQ ID NO: 3;
wherein the heterologous bundle sheath cell-preferred promoter comprises SEQ ID NO: 10.
4. The plant of claim 3 , wherein said glutaredoxin protein-encoding sequence comprises a sequence selected from the group of SEQ ID NOs:1 and 2, or encodes a protein selected from the group consisting of SEQ ID NOs:3-, 15-17, 18, 20, 21, 24-26 and 29.
5. Transformed seed of the plant of claim 3 , wherein said transformed seed comprises the said glutaredoxin protein-encoding sequence operably linked to the said promoter that is heterologous to said glutaredoxin protein-encoding sequence.
6. The plant of claim 3 wherein said plant is a monocot.
7. The plant of claim 3 wherein said plant is a dicot.
8. The method of claim 1 , wherein said glutaredoxin protein-encoding sequence is expressed from a bundle sheath cell-preferred promoter.
9. The plant of claim 3 , wherein said promoter that drives expression in a plant is a bundle sheath cell-preferred promoter.
10. A DNA construct comprising, in operable linkage,
a. A promoter that is functional in a plant cell, wherein the said promoter is a bundle sheath cell-preferred promoter which comprises SEQ ID NO: 10; and,
b. A nucleic acid sequence encoding a glutaredoxin protein which shares at least 80% sequence identity with SEQ ID NO: 3.
11. The DNA construct of claim 10 , wherein said nucleic acid sequence encoding a glutaredoxin protein comprises a sequence selected from the group of SEQ ID NOs:1 and 2, or encodes a protein selected from the group consisting of SEQ ID NOs:3, 15-17, 18, 20, 21, 24-26 and 29.
12. The DNA construct of claim 10 , wherein said promoter is heterologous to said nucleic acid sequence encoding a glutaredoxin protein.