Fusion proteins, recombinant bacteria, and exosporium fragments for plant health
Fusion proteins containing a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member are provided. The fusion proteins further comprise an enzyme having ACC deaminase activity, a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a glucanase, a phytase, an acid phosphatase, a pectinase, a mannanase, and/or an expansin protein. Also provided are recombinant Bacillus cereus family members that express the fusion proteins, exosporium fragments derived from the recombinant Bacillus cereus family members, and formulations containing the recombinant Bacillus cereus family members or exosporium fragments. Plant seeds treated with the recombinant Bacillus cereus family members, exosporium fragments, or formulations are also provided. The invention further relates to methods for stimulating plant growth and/or promoting plant health using the recombinant Bacillus cereus family members, exosporium fragments, or formulations.
1. A fusion protein comprising:
(A) a targeting sequence that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member; and
(B) a phospholipase C enzyme wherein the phospholipase C enzyme comprises: an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 373 or SEQ ID NO: 374 and having phospholipase C activity.
2. A recombinant Bacillus cereus family member comprising the fusion protein of claim 1 .
3. Exosporium fragments derived from the recombinant Bacillus cereus family of claim 2 .
4. A formulation comprising the recombinant Bacillus cereus family member of claim 2 or the exosporium fragments of claim 3 and an agriculturally acceptable carrier.
5. A method for stimulating plant growth and/or promoting plant health, comprising applying the recombinant Bacillus cereus family member of claim 2 or the exosporium fragments of claim 3 to a plant growth medium, a plant, a plant see, or an area surrounding a plant or a plant seed.
6. A method for delivering an enzyme to an animal, comprising feeding to the animal the recombinant Bacillus cereus family member of claim 2 , the exosporium fragments of claim 3 , or a combination thereof.
7. The fusion protein of claim 1 , wherein the phospholipase C enzyme further comprises a signal peptide.
8. The fusion protein of claim 7 , wherein the signal peptide is present at the amino terminus of the phospholipase C enzyme.
9. The fusion protein of claim 7 , wherein the phospholipase C enzyme further comprises a signal peptide at its amino terminus, wherein said signal peptide is selected from the group consisting of the amino acid sequence of SEQ ID NO: 340, SEQ ID NO: 339, SEQ ID NO: 334, SEQ ID NO: 335, SEQ ID NO: 336, SEQ ID NO: 337, SEQ ID NO: 338, and SEQ ID NO: 341.