Patent
The application is directed to a Lactobacillus plantarum strain, having the ability to generate a fermented milk presenting a high thickness and/or a high ropiness and/or a high thickness in mouth. The application also concerns a Lactobacillus plantarum strain, having the ability to generate a fermented milk presenting a high thickness and/or a high ropiness and/or a high thickness in mouth, further being a low post acidification strain at the temperature of fermentation. The application is also about a method to manufacture fermented product, in particular a fermented dairy product, using a L. plantarum strain of the invention or a bacterial composition, composition or kit-of part comprising a L. plantarum strain of the invention.
1. A method for manufacturing a fermented dairy product, wherein:
the method comprises:
a) inoculating a milk substrate with a Lactobacillus plantarum strain which is the strain DSM32493 deposited at the DSMZ on Apr. 26, 2017, or a variant of the DSM32493 strain deposited at the DSMZ on Apr. 26, 2017 with a genome sequence identity of at least 95% to the genome sequence of the DSM32493 strain, wherein the variant is produced by spontaneous mutation or genetic engineering of the DSM32493 strain, and
b) fermenting the inoculated milk substrate to obtain a fermented dairy product; and
the Lactobacillus plantarum strain is characterized as, when inoculated into milk, generating a fermented milk that exhibits the following rheological features, as assayed by test A:
a) a shear stress measured at shear rate 11.6 s-1 higher than 30 Pa,
b) a shear stress measured at shear rate 200 s-1 higher than 60 Pa, and
c) a difference of the shear stress measured at 146 s 1 minus the shear stress measured at 41.1 s-1 higher than 12.
2. The method according to claim 1 , wherein the Lactobacillus plantarum strain is further characterized by being a low post acidification strain at fermentation temperature.
3. The method according to claim 2 , wherein the Lactobacillus plantarum strain is mutated within the ATP-synthase operon.
4. The method of claim 3 , wherein the Lactobacillus plantarum strain bears a mutation in the ATP synthase alpha subunit gene of the ATP-synthase operon.
5. The method of claim 4 , wherein the Lactobacillus plantarum strain bears a mutation G to A at its position 506.
6. The method according to claim 1 , wherein the Lactobacillus plantarum strain is inoculated into the milk substrate in combination with at least one other lactic acid bacteria.
7. The method of claim 6 , wherein the other lactic acid bacteria is a Lactobacillus delbrueckii subsp bulgaricus strain.
8. The method according to claim 1 , wherein the Lactobacillus plantarum strain is inoculated together with a booster.
9. The method according to claim 8 , wherein the booster comprises a yeast extract or an amino-acid-containing composition.
10. The method according to claim 1 , wherein the variant has a genome sequence identity of at least 99% to the genome sequence of the DSM32493 strain.