Optogenetic probes for measuring membrane potential
Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.
1. A polypeptide comprising: a sequence of a modified microbial rhodopsin having at least 90% sequence identity to SEQ ID NO: 1; with one or more conservative substitutions comprising at least,
a first substitution at a position selected from the group consisting of D95 and D106; and
a second substitution at a position selected from the group consisting of P60, T80 and F161.
2. The polypeptide of claim 1 , wherein the one or more conservative substitutions is an amino acid substitution that maintains a structure of a backbone of the modified microbial rhodopsin.
3. The polypeptide of claim 1 , wherein the one or more conservative substitutions is an amino acid substitution that maintains the hydrophobicity of the sequence.
4. The polypeptide of claim 1 , wherein the one or more conservative substitutions is one of the following:
an alanine (A) substituted with an amino acid selected from the group consisting of D-ala, Gly, Aib, β-Ala, Acp, L-Cys and D-Cys;
an arginine (R) substituted with an amino acid selected from the group consisting of D-Arg, Lys, D-Lys, homo-Arg, D-homo-Arg, Met, Ile, D-Met, D-Ile, Orn and D-Orn;
an asparagine (N) substituted with an amino acid selected from the group consisting of D-Asn, Asp, D-Asp, Glu, D-Glu, Gln and D-Gln;
an aspartic acid (D) substituted with an amino acid selected from the group consisting of D-Asp, D-Asn, Asn, Glu, D-Glu, Gln and D-Gln;
a cysteine (C) substituted with an amino acid selected from the group consisting of D-Cys, S-Me-Cys, Met, D-Met, Thr and D-Thr;
a glutamine (Q) substituted with an amino acid selected from the group consisting of D-Gln, Asn, D-Asn, Glu, D-Glu, Asp and D-Asp;
a glutamic acid (E) substituted with an amino acid selected from the Group consisting D-Glu, D-Asp, Asp, Asn, D-Asn, Gln and D-Gln;
a glycine (G) substituted with an amino acid selected from the group consisting of Ala, D-Ala, Pro, D-Pro, Aib, β-Ala and Acp;
an isoleucine (I) substituted with an amino acid selected from the group consisting of D-Ile, Val, D-Val, AdaA, AdaG, Leu, D-Leu, Met and D-Met;
a leucine (L) substituted with an amino acid selected from the group consisting of D-Leu, Val, D-Val, AdaA, AdaG, Leu, D-Leu, Met and D-Met;
a lysine (K) substituted with an amino acid selected from the group consisting of D-Lys, Arg, D-Arg, homo-Arg, D-homo-Arg, Met, D-Met, Ile, D-Ile, Orn and D-Orn;
a methionine (M) substituted with an amino acid selected from the group consisting of D-Met, S-Me-Cys, Ile, D-Ile, Leu, D-Leu, Val and D-Val;
a phenylalanine (F) substituted with an amino acid selected from the group consisting of D-Phe, Tyr, D-Thr, L-Dopa, His, D-His, Trp, D-Trp, Trans-3,4 or 5-phenylproline, AdaA, AdaG, cis-3,4 or 5-phenylproline, Bpa and D-Boa;
a proline (P) substituted with an amino acid selected from the group consisting of D-Pro, L-I-thioazolidine-4-carboxylic acid and D-or-L-1-oxazolidine-4-carboxylic acid;
a serine (S) substituted with an amino acid selected from the group consisting of D-Ser, Thr, D-Thr, alto-Thr, Met, D-Met, Met (O), D-Met (O), L-Cys and D-Cys:
a threonine (T) substituted with an amino acid selected from the group consisting of D-Thr, Ser, D-Ser, allo-Thr, Met, D-Met, Met (O), D-Met (O), Val and D-Val;
a tyrosine (Y) substituted with an amino acid selected from the group consisting of D-Tyr, Phe, D-Phe, L-Dopa, His and D-His; or
a valine (V) substituted with an amino acid selected from the group consisting of D-Val, Leu, D-Leu, Ile, D-Ile, Met, D-Met, AdaA and AdaG.
5. The polypeptide of claim 1 , wherein the modified microbial rhodopsin comprises seven transmembrane domains.
6. The polypeptide of claim 1 , wherein the modified microbial rhodopsin comprises a protein core that binds a retinilydene chromophore.
7. The polypeptide of claim 1 , wherein the modified microbial rhodopsin comprises at least 96% sequence identity to SEQ ID NO: 1.
8. The polypeptide of claim 1 , wherein the modified microbial rhodopsin comprises at least 97% sequence identity to SEQ ID NO: 1.
9. The polypeptide of claim 1 , wherein the modified microbial rhodopsin comprises at least 98% sequence identity to SEQ ID NO: 1.
10. The polypeptide of claim 1 , wherein the modified microbial rhodopsin comprises, relative to SEQ ID NO: 1, at least a first substitution mutation selected from the group consisting of D95Q and D106H and a second substitution mutation selected from the group consisting of P60S, T80S, and F161V.
11. The polypeptide of claim 1 , wherein the sequence of the modified microbial rhodopsin is SEQ ID NO: 2.
12. The polypeptide of claim 1 , wherein the sequence of the modified microbial rhodopsin is SEQ ID NO: 3.
13. The polypeptide of claim 1 , wherein the sequence of the modified microbial rhodopsin comprises SEQ ID NO: 2.
14. The polypeptide of claim 1 , wherein the sequence of the modified microbial rhodopsin comprises SEQ ID NO: 3.
15. A polynucleotide encoding the polypeptide of claim 1 .