Humidity control in chemical reactors
Control of humidity in chemical reactors, and associated systems and methods, are generally described. In certain embodiments, the humidity within gas transport conduits and chambers can be controlled to inhibit unwanted condensation within gas transport pathways. By inhibiting condensation within gas transport pathways, clogging of such pathways can be limited (or eliminated) such that transport of gas can be more easily and controllably achieved. In addition, strategies for purging condensed liquid from chemical reactor systems are also described.
1. A reactor system, comprising:
a reactor chamber containing a liquid and at least one biological cell, and the reactor chamber has a volume of less than or equal to about 50 milliliters;
a reactor chamber gas outlet conduit configured to transport gas out of the reactor chamber through a reactor chamber gas outlet;
a flow control mechanism positioned in the gas outlet conduit and configured to regulate the flow of gas through the reactor chamber gas outlet conduit at a rate of equal to or less than about 1 milliliter per second; and
a liquid trap positioned in the gas outlet conduit and configured to remove liquid vapor from the gas within the reactor chamber gas outlet conduit, wherein the liquid trap is positioned in the gas outlet conduit between the flow control mechanism and the reactor chamber gas outlet.
2. The reactor system of claim 1 , further comprising:
a reactor chamber gas inlet conduit configured to transport gas into the reactor chamber, and
a humidifier configured to humidify the gas transported through the reactor chamber gas inlet conduit.
3. The reactor system of claim 2 , further comprising an inlet flow control mechanism configured to regulate the flow of gas through the reactor chamber gas inlet conduit at a flow rate of equal to or less than about 1 milliliter per second.
4. The reactor system of claim 3 , wherein the humidifier is positioned between the inlet flow control mechanism and the reactor chamber gas inlet.
5. The reactor system of claim 1 , wherein the at least one biological cell is a mammalian cell.
6. The reactor system of claim 5 , wherein the mammalian cell is selected from the group consisting of primate cells, bovine cells, horse cells, porcine cells, goat cells, dog cells, cat cells, rodent cells, human cells, and hamster cells.
7. The reactor system of claim 5 , wherein the mammalian cell is a Chinese hamster ovary (CHO) cell.
8. The reactor system of claim 5 , wherein the at least one biological cell is a cardiac cell, a fibroblast, a keratinocyte, a hepatocyte, a chondrocyte, a neural cell, a osteocyte, a muscle cell, a blood cell, an endothelial cell, an immune cell, or a stem cell.
9. The reactor system of claim 1 , wherein the gas comprises carbon dioxide and/or oxygen.
10. The reactor system of claim 2 , wherein the humidifier comprises a vessel including a first liquid having a temperature at least about 1° C. higher than the temperature of the liquid phase.
11. The reactor system of claim 1 , wherein the liquid trap comprises a vessel including a second liquid having a temperature at least about 1° C. lower than the temperature of the liquid phase.
12. The reactor system of claim 1 , wherein the flow control mechanism comprises a gas supply conduit comprising a constriction.
13. The reactor system of claim 12 , wherein a cross-sectional dimension of the gas supply conduit within the constriction is at least about 10% smaller than cross-sectional dimensions of the gas supply conduit at locations upstream and downstream of the constriction.
14. The reactor system of claim 1 , wherein the flow control mechanism comprises a pressure regulator.
15. The reactor system of claim 2 , wherein the humidifier is positioned upstream of the flow control mechanism.