1,1 ′-[[(substituted alkyl)imino]bis(alkylene)]bis- ferrocenes and their use in I electrochemical assays by labelling substrates of interest
Compounds of general formula I wherein Fc and Fc′ may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer, Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay.
1. A method of detecting a nucleic acid, the method comprising:
contacting the nucleic acid with a complementary nucleic acid probe under conditions to allow hybridization between said probe and an amplicon; and
selectively degrading the probe, wherein the probe is labelled with a compound selected from the group consisting of: 6-(bis((2-tert-butyl-sulfonylferrocenyl)1methylferrocenyl) amino)hexan-1-ol, 6-(bis((2-di-tert-butyl-phosphinyl-ferrocenyl)1-methylferrocenyl)amino)hexan-1-ol, 6-(bis((2-tert-butyl-sulfinylferrocenyl)1methylferrocenyl) amino)hexan-1-ol, and 6-(bis((2-tert-butyl-sulfidylferrocenyl)1methylferrocenyl) amino)hexan-1-ol.
2. The method of claim 1 , further comprising measuring electrochemical activity of the compound labelling the probe, wherein said electrochemical activity is dependent either quantitatively or qualitatively on the degradation of the probe.