Anti-B7-H3 antibodies and diagnostic uses thereof
Provided herein are B7-H3 antibodies, fragments of such antibodies, and compositions comprising the same. The antibodies, antibody fragments and compositions are useful in a number of analytical methods, including immunohistochemical and immunocytochemical detection and analysis of B7-H3. Also provided herein are isolated peptides and fusion proteins containing immunogenic determinants for said B7-H3 antibodies, animals immunized with the peptides and fusion proteins, isolated B cells obtained from the animals, and hybridomas made from the isolated B cells.
1. A composition comprising a fragment of human B7-H3 from 14 to 50 amino acids in length linked to a carrier protein, wherein the fragment of human B7-H3 comprises SEQ ID NO: 1 and the carrier protein is keyhole limpet haemocyanin (KLH).
2. The composition of claim 1 , wherein the fragment of human B7-H3 is from 14 to 40 amino acids in length.
3. The composition of claim 1 , wherein the fragment of human B7-H3 is from 14 to 30 amino acids in length.
4. The composition of claim 1 , wherein the fragment of human B7-H3 is from 14 to 25 amino acids in length.
5. The composition of claim 1 , wherein the fragment of human B7-H3 is from 14 to 20 amino acids in length.
6. The composition of claim 1 , wherein the fragment of human B7-H3 consists essentially of SEQ ID NO: 1.
7. The composition of claim 1 , wherein the fragment of human B7-H3 consists of SEQ ID NO: 1.
8. An isolated B cell capable of producing an antibody that specifically binds to SEQ ID NO: 1, wherein said isolated B-cell is obtained by a method comprising:
(a) immunizing an animal with a fragment of human B7-H3 from 14 to 50 amino acids in length linked to a carrier protein, wherein the fragment of human B7-H3 comprises SEQ ID NO: 1, wherein the carrier protein is keyhole limpet haemocyanin (KLH), and wherein the animal is a rabbit, rat, mouse, or goat;
(b) isolating antibody-expressing B-cells from the animal; and
(c) screening the antibody-producing B-cells for the presence of a B-cell that produces the antibody that specifically binds to SEQ ID NO: 1.
9. The isolated B cell of claim 8 , wherein the fragment of human B7-H3 is from 14 to 40 amino acids in length.
10. The isolated B cell of claim 8 , wherein the fragment of human B7-H3 is from 14 to 30 amino acids in length.
11. The isolated B cell of claim 8 , wherein the fragment of human B7-H3 is from 14 to 25 amino acids in length.
12. The isolated B cell of claim 8 , wherein the fragment of human B7-H3 is from 14 to 20 amino acids in length.
13. The isolated B cell of claim 8 , wherein the fragment of human B7-H3 consists essentially or consists of SEQ ID NO: 1.
14. A hybridoma capable of producing an antibody that specifically binds to SEQ ID NO: 1, wherein the hybridoma is obtained by a method comprising:
(a) immunizing an animal with a fragment of human B7-H3 from 14 to 50 amino acids in length linked to a carrier protein, wherein the fragment of human B7-H3 comprises SEQ ID NO: 1, wherein the carrier protein is keyhole limpet haemocyanin (KLH), and wherein the animal is a rabbit, rat, mouse, or goat;
(b) isolating antibody-producing B-cells from the animal;
(c) screening the antibody-producing B-cells for the presence of a B-cell that produces an antibody that specifically binds to SEQ ID NO: 1; and
(d) preparing the hybridoma from the B-cell that produces the antibody that specifically binds to SEQ ID NO: 1.
15. The hybridoma of claim 14 , wherein the fragment of human B7-H3 is from 14 to 40 amino acids in length.
16. The hybridoma of claim 14 , wherein the fragment of human B7-H3 is from 14 to 30 amino acids in length.
17. The hybridoma of claim 14 , wherein the fragment of human B7-H3 is from 14 to 25 amino acids in length.
18. The hybridoma of claim 14 , wherein the fragment of human B7-H3 is from 14 to 20 amino acids in length.
19. The hybridoma of claim 14 , wherein the fragment of human B7-H3 consists essentially of or consists of SEQ ID NO: 1.