Compositions comprising ZDHHC1 DNA in a complex
Provided herein is technology relating compositions and methods for detecting tissue cell-specific DNA, such as epithelial cell-specific DNA, in blood or blood products from a subject. The technology also relates to use of tissue cell-specific DNAs as internal controls for methylation assays.
1. A composition comprising a complex, the complex comprising:
i) a target sequence-specific capture reagent comprising a particle covalently attached to a target-specific capture probe oligonucleotide complementary to methylated human ZDHHC1 DNA at a site within SEQ ID NO:26 or its complement, and
ii) a strand of methylated ZDHHC1 DNA comprising SEQ ID NO:26 or its complement from a sample from a human subject, wherein cytosine-phosphate-guanine (CpG) dinucleotides in the methylated ZDHHC1 DNA are methylated;
wherein the target-specific capture probe oligonucleotide is specifically hybridized to the strand of methylated ZDHHC1 DNA at a site within SEQ ID NO:26 or its complement.
2. The composition of claim 1 , wherein the particle is a magnetic particle.
3. The composition of claim 1 , wherein the target-specific capture probe oligonucleotide has a complete length of between 15 and 100 nucleotides.
4. The composition of claim 3 , wherein the complete length of the target-specific capture probe oligonucleotide is hybridized to the methylated ZDHHC1 DNA within SEQ ID NO:26.
5. The composition of claim 1 , wherein the target-specific capture probe oligonucleotide is covalently attached to the particle by a non-nucleic acid chemical linkage.
6. The composition of claim 5 wherein the non-nucleic acid chemical linkage comprises a multi-carbon chain.
7. The composition of claim 6 , wherein the multi-carbon chain is attached to a 5′ nucleotide of the target-specific capture probe oligonucleotide.
8. The composition of claim 1 , wherein the target-specific capture probe oligonucleotide comprises SEQ ID NO:36.
9. The composition of claim 1 , further comprising a clarified supernatant from a stool sample.
10. The composition of claim 9 , wherein the clarified supernatant comprises guanidine thiocyanate.
11. The composition of claim 9 , wherein the clarified supernatant is produced by a process comprising treating a stool supernatant to remove assay inhibitors.
12. The composition of claim 9 , wherein the clarified supernatant is produced by a process comprising treating a stool supernatant with polyvinylpolypyrrolidone.