Process for producing at least one metabolite of interest by conversion of a pentose in a microorganism
Disclosed is a process for producing at least one metabolite of interest by conversion of a pentose in a microorganism. The process includes at least: (i) an operation of culturing a recombinant microorganism expressing a synthetic pathway for pentose assimilation which includes at least the following steps: a) phosphorylation in position 1 of a pentose chosen from (D)-xylulose and/or (L)-ribulose, b) cleavage of the pentose-1-phosphate obtained at the end of step a), in order to obtain glycolaldehyde and dihydroxyacetone phosphate (DHAP), and (ii) an operation of recovering the at least one metabolite of interest obtained at the end of the culturing operation (i). Also disclosed is an associated microorganism.
1. Process for producing at least one of ethylene glycol and glycolic acid by converting pentose in a microorganism, said process comprising:
(i) an operation of culturing a recombinant microorganism expressing a synthetic pathway for pentose assimilation, the operation comprising at least the following steps:
a) phosphorylation in position 1 of a pentose comprising at least one of (D)-Xylulose, and (L)-Ribulose, wherein the phosphorylation is catalyzed by a recombinantly expressed kinase from said synthetic pathway, capable to phosphorylate in position 1 a pentose selected from (D)-Xylulose and/or (L)-Ribulose,
b) cleavage of the pentose-1-phosphate obtained at the end of step a) in order to obtain glycolaldehyde and dihydroxyacetone phosphate (DHAP), wherein the cleavage is catalyzed by a recombinantly expressed aldolase from said synthetic pathway, capable to cleavage the pentose-1-phosphate in order to obtain glycolaldehyde and dihydroxyacetone phosphate (DHAP),
and at least one of the following steps:
c) reduction of the glycolaldehyde obtained at the end of step b) to ethylene glycol, wherein the reduction is catalyzed by a recombinantly expressed glycolaldehyde reductase from said synthetic pathway, capable to catalyze reduction of the glycolaldehyde obtained at the end of step b) to ethylene glycol, and
c′) oxidation of the glycolaldehyde obtained at the end of step b) to glycolic acid, wherein the oxidation step c′) is catalyzed by a recombinantly expressed glycolaldehyde dehydrogenase from said synthetic pathway, capable to catalyze an oxidation of the glycolaldehyde obtained at the end of step b) to glycolic acid,
(ii) an operation of recovering said at least on of ethylene glycol and glycolic acid obtained at the end of the culturing operation (i).