Heat-treated limulus amebocyte lysates
The application provides heat-treated Limulus amebocyte lysates useful for detecting β-glucans.
1. A heat-treated Limulus amebocyte lysate comprising:
i) (1→3)-(β-D-glucan sensitive Limulus Factor G and
ii) Factor C having an endotoxin reactivity less than 0.1% of the endotoxin reactivity of an untreated Limulus amebocyte lysate.
2. The Limulus amebocyte lysate of claim 1 , wherein the Limulus amebocyte lysate is lyophilized.
3. The Limulus amebocyte lysate of claim 1 , wherein the Limulus amebocyte lysate retains sensitivity to 1 pg/mL carboxymethylated pachyman.
4. The Limulus amebocyte lysate of claim 3 , wherein the Limulus amebocyte lysate retains sensitivity to 0.1 pg/mL carboxymethylated pachyman.
5. A Limulus amebocyte lysate according to claim 1 , wherein the lysate is insensitive to 200 EU/mL of Reference Standard Endotoxin.
6. A Limulus amebocyte lysate according to claim 1 , wherein the lysate is insensitive to 0.01 mcg/mL LPS.
7. The Limulus amebocyte lysate of claim 6 , wherein the lysate is insensitive to 0.1 mcg/mL LPS.
8. The Limulus amebocyte lysate of claim 7 , wherein the lysate is insensitive to 1 mcg/mL LPS.
9. The Limulus amebocyte lysate of claim 8 , wherein the lysate is insensitive to 10 mcg/mL LPS.
10. A composition comprising the heat-treated Limulus amebocyte lysate of claim 1 and a substrate.
11. The composition of claim 10 , wherein the substrate is chromogenic.
12. The composition of claim 10 , wherein the substrate is fluorogenic.
13. The composition of claim 10 , wherein the composition is lyophilized.
14. The Limulus amebocyte lysate of claim 1 , wherein the heat-treated Limulus amebocyte lysate comprises inactive Factor C.