Methods of obtaining androecious cucurbit plants
Disclosed is a method for the production of a plant of the Cucurbitaceae family by mutating seeds to induce a mutation of 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8) and growing a plant from the mutated seed, with the plant having a androecy or androecious tendency.
1. A method for the production of androecious or of androecious tendency plant of the Cucurbitaceae family selected from the group consisting of Cucumis, Citrullus, Luffa, Momordica and Lagenaria genera, with the exclusion of the Cucumis sativus species, comprising the steps of
i) exposing seed of the Cucurbitaceae family selected from the group consisting of Cucumis, Citrullus, Luffa, Momordica and Lagenaria genera, with the exclusion of the Cucumis sativus species, to a mutagenic agent, wherein the mutagenic agent induces one or more non-natural mutations in at least one 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8) allele in the genome of the seed, and
ii) growing a plant from the seed obtained in step i), wherein the plant comprises at least one mutated 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8) allele thus conferring androecy or androecious tendency, wherein the presence of the at least one mutated ACS8 allele is determined by an absence or a reduced enzymatic activity of at least 50% of SEQ ID NO:12, SEQ ID NO:21, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:36 compared to a plant of the same species grown from a seed that was not exposed to the mutagenic agent, and wherein the at least one mutated ACS8 allele encodes an ACS8 polypeptide having at least 95% sequence identity with SEQ ID NO:12, SEQ ID NO:21, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:36.
2. The method of claim 1 , further comprising self-fecundating of the plant of the Cucurbitaceae family grown from the mutated seed to obtain a collection of mutant plants.
3. The method of claim 1 , further comprising selecting the plant of the Cucurbitaceae family having the at least one mutated ACS8 allele encoding an ACS8 polypeptide having at least 95% sequence identity with SEQ ID NO:12, SEQ ID NO:21, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:36 and which are androecious or of androecious tendency.
4. The method of claim 3 , further comprising crossing two selected plants of the Cucurbitaceae family selected from the group consisting of Cucumis, Citrullus, Luffa, Momordica and Lagenaria genera, with the exclusion of the Cucumis sativus species, having the at least one mutated ACS8 allele encoding an ACS8 polypeptide having at least 95% sequence identity with SEQ ID NO:12, SEQ ID NO:21, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:36, and further selecting a plant homozygous for the mutated ACS8 allele that can be used as a pollinator.
5. The method of claim 1 , wherein the mutagenic agent is ethylmethanesulfone (EMS).
6. The method of claim 4 , wherein the two crossed plants of the Cucurbitaceae family are from the same species.