Chimeric canine anti-CD20 antibody
The disclosure relates to caninized chimeric anti-CD20 antibodies to the canine protein CD20 and methods of use to treat certain disorders such as non-Hodgkins B-cell lymphoma in dogs.
1. An antibody comprising:
a light chain variable region (LCVR) comprising a first complementary determining region (LCDR1) whose amino acid sequence is that given in SEQ ID NO: 14, a second CDR (LCDR2) whose amino acid sequence is that given in SEQ ID NO: 16, a third CDR (LCDR3) whose amino acid sequence is that given in SEQ ID NO: 18, and a joining (J) region whose amino acid sequence is that given in SEQ ID NO: 19; and
a heavy chain variable region (HCVR) comprising a first CDR (HCDR1) whose amino acid sequence is that given in SEQ ID NO: 5, a second CDR (HCDR2) whose amino acid sequence is that given in SEQ ID NO: 7, and a third CDR (HCDR3) whose amino acid sequence is that given in SEQ ID NO: 9; and wherein the antibody specifically binds to canine CD20.
2. The antibody of claim 1 , wherein the antibody comprises two light chains (LC) each having a LCDR1 of SEQ ID NO: 14, a LCDR2 of SEQ ID NO: 16, a LCDR3 of SEQ ID NO: 18, and a J region of SEQ ID NO: 19; and two heavy chains (HC) each having a HCDR1 of SEQ ID NO: 5, a HCDR2 of SEQ ID NO: 7, and a HCDR3 of SEQ ID NO: 9.
3. A method of treating lymphoma in a canine patient, comprising administering to a lymphoma cancer canine patient in need of such treatment an effective amount of an antibody comprising a light chain variable region (LCVR) comprising a LCDR1 of SEQ ID NO: 14, a LCDR2 of SEQ ID NO: 16, a LCDR3 of SEQ ID NO: 18, and a J region of SEQ ID NO: 19; and a heavy chain variable region (HCVR) comprising a HCDR1 of SEQ ID NO: 5, a HCDR2 of SEQ ID NO: 7, and a HCDR3 of SEQ ID NO: 9; wherein the antibody specifically binds to canine CD20.
4. A kit, comprising a pharmaceutical composition, comprising an antibody which specifically binds to canine CD20, with one or more pharmaceutically acceptable carriers, diluents, or excipients;
wherein the antibody which specifically binds to canine CD20 comprises a light chain variable region (LCVR) comprising a LCDR1 of SEQ ID NO: 14, a LCDR2 of SEQ ID NO: 16, a LCDR3 of SEQ ID NO: 18, and a J region of SEQ ID NO: 19; and a heavy chain variable region (HCVR) comprising a HCDR1 of SEQ ID NO: 5, a HCDR2 of SEQ ID NO: 7, and a HCDR3 of SEQ ID NO: 9.