METHODS AND COMPOSITIONS FOR SUPERPARAMAGNETIC PARTICLES FOR NUCLEIC ACID EXTRACTION
A composition is described comprising superparamagnetic particles. Optionally, the particles comprise microbeads. Optionally, the particles comprise nanobeads. Optionally, the particles are non-spherical particles. Optionally, the particles are non-spherical particles suitable for RNA or DNA extraction. A method is described for forming microparticles. Optionally, the method comprises using citrate precipitation. Optionally, a kit comprising one or more of the particles is described. Optionally, a kit for sample preparation for nucleic acid extraction is described comprising non-spherical microparticles or nanoparticles.
1 . A composition comprising superparamagnetic particles.
2 . The composition of claim 1 , wherein said particles comprise microbeads.
3 . The composition of claim 1 , wherein said particles comprise nanobeads.
4 . The composition of claim 1 , wherein said particles comprise sphere-like magnetic aggregate with a size range of about 1 to about 2 microns.
5 . A method of forming microparticles.
6 . The method of claim 4 , using citrate precipitation.
7 . A kit comprising one or more of the particles of claim 1 .
8 . A kit comprising one or more of the particles of claim 2 .
9 . A kit comprising one or more of the particles of claim 3 .
10 - 15 . (canceled)
16 . The method of claim 5 wherein said microparticles are sphere-like particles each with a size range of about 1 to about 2 microns.
17 . The method of claim 5 wherein said microparticles each comprise a metal core coated with a polymer.
18 . The method of claim 5 wherein said microparticles each comprise a metal core coated with a silica.
19 . The method of claim 5 comprising using a one-pot two-step process, wherein a first step comprises using ammonia neutralization of metal particles and a second step comprises using a citrate salt to stabilize the metal particles.
20 . The method of claim 19 further comprises silica coating said microparticles by hydrolysis of tetraethoxysilane (TEOS) under alkaline condition.