Methods and systems for purifying non-complexed
Methods and systems for chromatographically purifying a botulinum neurotoxin are provided. These methods and systems allow for efficient purification of a non-complexed form of the botulinum neurotoxin in high purity and yield that can be used as an active ingredient in pharmaceutical preparations.
1. A method for purifying a non-complexed botulinum toxin, the method comprising:
(i) providing a mixture comprising a crude non-complexed botulinum toxin;
(ii) loading the mixture on an anion exchange column so as to permit capture of the non-complexed botulinum toxin by the anion exchange column;
(iii) eluting the non-complexed botulinum toxin from the anion exchange column to give an eluent comprising the non-complexed botulinum toxin;
(iv) loading a cation exchange column with the eluent from the anion exchange column so as to permit capture of the non-complexed botulinum toxin by the cation exchange column; and
(v) eluting purified non-complexed botulinum toxin from the cation exchange column.
2. The method according to claim 1 , wherein the crude non-complexed botulinum toxin is obtained by
obtaining a sample comprising botulinum toxin complex;
loading a hydrophobic interaction column with the sample so as to permit capture of the botulinum toxin complex by the hydrophobic interaction column;
eluting the botulinum toxin complex from the hydrophobic interaction chromatography column; and
dissociating the botulinum toxin complex to obtain the mixture comprising the crude non-complexed botulinum toxin.
3. The method according to claim 2 , wherein the sample is a cell supernatant or filtrate comprising the botulinum toxin complex.
4. The method according to claim 2 , wherein the sample comprising a botulinum toxin complex is obtained by:
subjecting a fermentation culture comprising the botulinum toxin to acid precipitation to obtain an acid precipitate; and
performing tangential flow filtration on the precipitate to concentrate precipitate.
5. The method according to claim 2 , wherein the sample comprising a botulinum toxin complex is obtained by subjecting an insoluble fraction of a fermentation culture to tangential flow filtration.
6. The method according to claim 2 , wherein the sample comprising a botulinum toxin complex is subjected to a nuclease digestion before loading on the hydrophobic interaction column.
7. The method according to claim 6 , wherein the nuclease is derived from an animal product free process.
8. The method according to claim 1 , wherein the method is animal product free.
9. The method according to claim 1 , wherein the purified non-complexed botulinum toxin comprises a botulinum toxin type A.
10. The method according to claim 1 , wherein the purified non-complexed botulinum toxin is at least 95% pure.
11. The method according to claim 1 , wherein the purified non-complexed botulinum toxin has an activity of at least 200 LD50 units/ng.
12. The method according to claim 1 , wherein the method produces a yield of at least about 2 mg/L fermentation culture.
13. The method according to claim 1 wherein the anion column comprises a chromatography gel matrix comprising beaded agarose having a net negative charge, and wherein the cation column comprises a chromatography gel matrix comprising beaded agarose having a net positive charge; wherein said anion and cation exchange columns provide separation of proteins based on electrostatic charge.
14. The method according to claim 1 wherein a buffer for loading the non-complexed botulinum toxin onto the anionic column is selected from the group consisting of Tris, bis-Tris, triethanolamine, and N-methyl diethanolamine.
15. The method according to claim 14 wherein the buffer is used at a pH from 7.4 to 8.2.
16. The method according to claim 1 wherein a buffer for loading the non-complexed botulinum toxin onto the cationic column is selected from the group consisting of sodium phosphate, MES, and HEPES.
17. The method according to claim 16 wherein the buffer is used at a pH from 6.0 to 7.0.
18. The method according to claim 1 wherein pH of the anionic column is from 7.4 to 8.2.
19. The method according to claim 1 wherein pH of the cationic column is from 6.0 to 7.0.
20. The method according to claim 1 wherein a gradient for eluting the non-complexed botulinum toxin from the anionic column is selected from the group consisting of an ascending gradient of sodium chloride and an ascending gradient of potassium chloride.
21. The method according to claim 20 wherein the gradient is used at a pH from 7.4 to 8.4.
22. The method according to claim 1 wherein a gradient for eluting the non-complexed botulinum toxin from the cationic column is selected from the group consisting of an ascending gradient of sodium chloride and an ascending gradient of potassium chloride.
23. The method according to claim 22 wherein the gradient is used at a pH from 6.0 to 7.0.