Method for treatment and control of plant disease
Methods and compositions are provided for preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas axonopodis in a plant. The invention provides novel bacteriophages virulent to Xylella fastidiosa or Xanthomonas axonopodis , including XfaMija and XfaMijo, and further provides methods for treating or preventing Pierce's Disease or Citrus Canker in plants.
1. A method of preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas in a plant, comprising contacting said plant with particles of at least one virulent bacteriophage, wherein Xylella fastidiosa and/or Xanthomonas axonopodis are hosts of the bacteriophage, selected from the group consisting of bacteriophages of the Xfas100 phage type, bacteriophages of the Xfas300 phage type, and bacteriophages of the Xfas500 type; wherein the Xfas100 phage type comprises a genome with a DNA sequence 90% or more identical to a sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18; the Xfas300 phage type comprises a genome with a DNA sequence 90% or more identical to a sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ.ID 22, SEQ ID NO:23, and SEQ ID NO:24; and wherein the Xfas500 phage type comprises a DNA sequence selected from the group consisting of SEQ ID NO:25-28.
2. The method of claim 1 , wherein said bacteriophage is capable of lysing said Xylella fastidiosa and/or Xanthomonas axonopodis bacteria.
3. The method of claim 1 , wherein said bacteriophage infects a cell by binding to a Type IV pilus.
4. The method of claim 1 , wherein contacting comprises introducing the bacteriophage particles into the plant.
5. The method of claim 1 , wherein the plant is selected from the group consisting of a grapevine plant, a citrus plant, almond, coffee, alfalfa, oleander , oak, sweetgum, redbud, elm, peach, apricot, plum, blackberry, mulberry, olive, and Chitalpa tashkentensis.
6. The method of claim 4 , wherein the bacteriophage particles are introduced into the plant by injection, by an insect vector, via the root system, by spray, by mist, or by dust on the plant.
7. The method of claim 6 , wherein the insect vector is a glassy winged sharpshooter.
8. The method of claim 4 , wherein the number of said bacteriophage particles to be introduced into said plant is from 1 to 10 12 PFU/ml.
9. The method of claim 4 , wherein said bacteriophage particles are introduced simultaneously or sequentially to the plant by a combination of two, three, four, five, or six virulent bacteriophage strains.
10. The method of claim 1 for preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas in a plant, comprising contacting said plant with particles of at least one virulent bacteriophage, wherein Xylella fastidiosa and/or Xanthomonas axonopodis are hosts of the bacteriophage; wherein the Xfas500 phage type bacteriophage have been deposited under ATCC Accession Numbers ATCC PTA-122743 and ATCC PTA-122742, or variants thereof; wherein the Xfas100 phage type is at least one bacteriophage selected from the group consisting of: Xfas103, and Xfas106; and the Xfas300 phage type is at least one bacteriophage selected from the group consisting of: Xfas302, Xfas303, Xfas304, and Xfas306; wherein said phage of the Xfas100 and Xfas300 phage types have been deposited under ATCC Accession Numbers PTA-13096, PTA-13095, PTA-13098, PTA-13099, PTA-13100, and PTA-13097, respectively, for phages Xfas103, Xfas106, Xfas302, Xfas303, Xfas304, and Xfas306.
11. The method of claim 1 , wherein the Xfas100 phage type comprises a genome with a DNA sequence 95% or more identical to a sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18; and the Xfas300 phage type comprises a genome with a DNA sequence 95% or more identical to a sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ.ID 22, SEQ ID NO:23, and SEQ ID NO:24.
12. The method of claim 1 , wherein the Xfas 100 phage type comprises a genome with a DNA sequence 98% or more identical to a sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18; and the Xfas300 phage type comprises a genome with a DNA sequence 98% or more identical to a sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ.ID 22, SEQ ID NO:23, and SEQ ID NO:24.
13. The method of claim 1 , wherein the Xfas 100 phage type comprises a genome with a DNA sequence 99% or more identical to a sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18; and the Xfas300 phage type comprises a genome with a DNA sequence 99% or more identical to a sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ.ID 22, SEQ ID NO:23, and SEQ ID NO:24.