Recombinant arterivirus replicon systems and uses thereof
The present disclosure generally relates to viral-based expression systems suitable for the production of molecule of interests in recombinant host cells. The disclosure particularly relates to nucleic acid constructs, such as expression vectors, containing a modified arterivirus genome or replicon RNA in which at least some of its original viral sequence has been deleted. Also included in the disclosure are viral-based expression vectors including one or more expression cassettes encoding heterologous polypeptides. In some embodiments, the expression cassettes are configured and positioned at defined locations on the viral genome so as to enable expression of the heterologous polypeptides in a tunable manner.
1. A method for producing a polypeptide of interest, comprising culturing a host cell comprising a nucleic acid, wherein the nucleic acid comprises a gene encoding the polypeptide of interest and a nucleotide sequence encoding a modified Equine arteritis virus (EAV) genome or replicon RNA, wherein the modified EAV genome or replicon RNA comprises a sequence fragment exhibiting at least 80% sequence identity to the sequence encoding open reading frame ORF7 of the wild-type sequence of EAV, and wherein the modified genome or replicon RNA is devoid of the sequence encoding ORF2a, and further wherein the polypeptide of interest is expressed.
2. A method for producing a polypeptide of interest in a subject, comprising administering to the subject a nucleic acid, wherein the nucleic acid comprises:
i. a nucleotide sequence encoding a modified Equine arteritis virus (EAV) genome or replicon RNA, wherein the modified EAV genome or replicon RNA comprises a sequence fragment exhibiting at least 80% sequence identity to the sequence encoding open reading frame ORF7 of the wild-type sequence of EAV, and wherein the modified EAV genome or replicon RNA is devoid of the sequence encoding ORF2a; and
ii. one or more expression cassettes, wherein each of the one or more expression cassettes comprises a subgenomic (sg) promoter operably linked to a heterologous nucleotide sequence encoding the polypeptide of interest;
and wherein the polypeptide of interest is expressed.
3. A composition comprising a nucleic acid, wherein the nucleic acid comprises a nucleotide sequence encoding a modified Equine arteritis virus (EAV) genome or replicon RNA, further wherein the nucleic acid comprises a gene encoding a polypeptide of interest, wherein the modified EAV genome or replicon RNA comprises a sequence fragment exhibiting at least 80% sequence identity to the sequence encoding open reading frame ORF7 of the wild-type sequence of EAV, and wherein the modified EAV genome or replicon RNA is devoid of the sequence encoding ORF2a; and a pharmaceutically acceptable carrier.
4. A composition comprising a recombinant cell comprising a nucleic acid, wherein the nucleic acid comprises a nucleotide sequence encoding a modified Equine arteritis virus (EAV) genome or replicon RNA, further wherein the nucleic acid comprises a sequence encoding a gene of interest (GOI), wherein the modified EAV genome or replicon RNA comprises a sequence fragment exhibiting at least 80% sequence identity to the sequence encoding open reading frame ORF7 of the wild-type sequence of EAV and wherein the modified EAV genome or replicon RNA is devoid of the sequence encoding ORF2a, and a pharmaceutically acceptable carrier.
5. The method of claim 1 , wherein the modified EAV genome or replicon RNA is devoid of an ATG start codon of ORF7 and/or ORF6, or comprises an inactivated ATG start codon of ORF7 and/or ORF6.
6. The method of claim 2 , wherein the modified EAV genome or replicon RNA is devoid of an ATG start codon of ORF7 and/or ORF6, or comprises an inactivated ATG start codon of ORF7 and/or ORF6.
7. The composition of claim 3 , wherein the modified EAV genome or replicon RNA is devoid of an ATG start codon of ORF7 and/or ORF6, or comprises an inactivated ATG start codon of ORF7 and/or ORF6.
8. The composition of claim 4 , wherein the modified EAV genome or replicon RNA is devoid of an ATG start codon of ORF7 and/or ORF6, or comprises an inactivated ATG start codon of ORF7 and/or ORF6.
9. The method of claim 2 , wherein the modified EAV genome or replicon RNA comprises a 5′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 1 and a 3′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 2.
10. The method of claim 9 , wherein the 5′ region comprises SEQ ID NO: 1 and the 3′ region comprises SEQ ID NO: 2.
11. The method of claim 2 , wherein the modified EAV genome or replicon RNA comprises a 5′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 40 and a 3′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 41.
12. The method of claim 11 , wherein the 5′ region comprises SEQ ID NO: 40 and the 3′ region comprises SEQ ID NO: 41.
13. The method of claim 2 , wherein the sg promoter comprises a sequence exhibiting at least 80% sequence identity to a sequence selected from the group consisting of sg promoter 1, sg promoter 2, sg promoter 3, sg promoter 4, sg promoter 5, sg promoter 6, and sg promoter 7.
14. The method of claim 2 , wherein the nucleic acid further comprises one or more spacer regions operably positioned adjacent to the sg promoter.
15. A method for producing a polypeptide of interest, comprising culturing a host cell comprising a nucleic acid, wherein the nucleic acid comprises a gene encoding the polypeptide of interest and a modified Equine arteritis virus (EAV) sub-genomic replicon RNA, wherein the modified EAV sub-genomic replicon RNA comprises a sequence fragment exhibiting at least 80% sequence identity to the sequence encoding open reading frame ORF7 of the wild-type sequence of EAV and further comprises a nucleotide sequence encoding at least one of (a) ORF1a and ORF1b; (b) a 5′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 1 and a 3′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 2; and (c) a 5′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 40 and a 3′ region comprising a nucleotide sequence exhibiting at least 80% sequence identity to SEQ ID NO: 41; and wherein the modified sub-genomic replicon RNA is devoid of the sequence encoding ORF2a, and further wherein the polypeptide of interest is expressed.
16. The method of claim 15 , wherein the 5′ region comprises SEQ ID NO: 1 and the 3′ region comprises SEQ ID NO: 2.
17. The method of claim 15 , wherein the 5′ region comprises SEQ ID NO: 40 and the 3′ region comprises SEQ ID NO: 41.