Bacterial Growth on Non-Animal Derived Media
The invention is directed to tools, compositions, and methods for the cultivation of microorganisms in culture media that is devoid of animal-derived materials such as blood, and, in particular, to compositions of meat-free media.
1 . A composition comprising one or more salts; a magnesium salt; a calcium salt; soy meal; a saccharide; at least two amino acids; yeast extract; a ferrous or ferric salt; and pyruvate, wherein the composition contains no animal products.
2 . The composition of claim 1 , wherein the one or more salts comprises sodium chloride.
3 . The composition of claim 1 , wherein the magnesium salt comprises magnesium chloride or magnesium sulfate.
4 . The composition of claim 1 , wherein the calcium salt comprises calcium chloride or calcium sulfate.
5 . The composition of claim 1 , wherein the soy meal comprises an enzymatic hydrolysate of soy meal.
6 . The composition of claim 1 , wherein the at least two amino acids comprise cysteine and thiamine.
7 . The composition of claim 1 , wherein the saccharide comprise glucose.
8 . The composition of claim 1 , wherein the ferrous or ferric salt comprise ferrous sulfate or ferric citrate.
9 . The composition of claim 1 , which is an aqueous solution or a dry powder.
10 . The composition of claim 9 , wherein the aqueous solution comprises from about 1-5 g/L of the one or more salts; from about 0.1 to 2.0 g/L of the magnesium salt; from about 0.001 to 0.1 g/L of the calcium salt; from about 2-10 g/L of the soy meal; from about 5-20 g/L of the saccharide; from about 0.001 to 0.1 g/L of the at least two amino acids; from about 1-10 g/L of the yeast extract; from about 0.0001-0.001% of the ferrous or ferric salt; and from about 0.01-1.0% of the pyruvate.
11 . The composition of claim 1 , wherein the animal products excluded are obtained or derived from a mammal.
12 . The composition of claim 1 , wherein the products excluded that are obtained or derived from a mammal are fetal bovine serum, bovine serum, caprine serum, and/or equine serum.
13 . A method for culturing a microorganism comprising:
obtaining a sample of the microorganism; and
contacting the microorganism to a medium comprising the composition of claim 1 .
14 . The method of claim 13 , wherein the microorganism comprises Streptococcus pneumoniae.
15 . A composition comprising one or more salts; soy meal; a saccharide; yeast extract; a plant protein hydrolysate, a ferrous or ferric salt; and pyruvate, wherein the composition contains no animal products.
16 . The composition of claim 15 , wherein the one or more salts comprises sodium chloride.
17 . The composition of claim 15 , wherein the soy meal comprises an enzymatic hydrolysate of soy meal.
18 . The composition of claim 15 , wherein the saccharide comprise glucose.
19 . The composition of claim 15 , wherein the yeast extract comprises a vegetable yeast extract.
20 . The composition of claim 15 , wherein the plant protein hydrolysate comprises atholate.
21 . The composition of claim 15 , wherein the ferrous or ferric salt comprise ferrous sulfate or ferric citrate.
22 . The composition of claim 15 , which is an aqueous solution or a dry powder.
23 . The composition of claim 22 , wherein the enzymatic hydrolysate of soy meal is at a concentration of from about 0.5-10%, the polysaccharide is at a concentration of from about 0.5-5%, the vegetable yeast extract is at a concentration of from about 0.1-10%, the plant protein hydrolysate is at a concentration of from about 1-10%, the ferrous or ferric salt is at a concentration of from about 0.001-0.01%, and the pyruvate is at a concentration of from about 0.01-1.0%.
24 . A method for culturing a microorganism comprising:
obtaining a sample of the microorganism; and
contacting the microorganism to a medium comprising the composition of claim 15 .
25 . The method of claim 24 , wherein the microorganism comprises Streptococcus pneumonia.