Method for Enhancing Passenger Molecule Loading
A composition comprising surfactant-enhanced phospholipid vesicles with one or more cannabinoid substance encapsulated therein is disclosed, wherein one or more surfactant is utilized for enhancing loading and increasing encapsulation efficiency of cannabinoid passenger molecules within phospholipid structures. A method is disclosed for making a surfactant-enhanced phospholipid vesicles with one or more cannabinoid substance encapsulated therein, wherein one or more surfactant is used for enhancing loading and increasing encapsulation efficiency of passenger molecules in phospholipid structures. A method of using surfactant-enhanced phospholipid vesicles with one or more cannabinoid substance encapsulated therein is disclosed wherein one or more surfactant enhances loading and increases encapsulation efficiency of cannabinoid substances in phospholipid structures. A composition and method of making surfactant-enhanced phospholipid vesicles with one or more lipophilic passenger substance encapsulated therein is disclosed wherein one or more surfactant is utilized for enhancing loading and increasing encapsulation efficiency of passenger molecules within phospholipid structures.
1 . A method of making a surfactant-enhanced phospholipid vesicle encapsulating one or more cannabinoid substance comprising the steps of:
providing a cannabinoid substance having one or more cannabinoid compound, wherein one of said one or more cannabinoid compound is cannabidiol (CBD);
providing a phospholipid stock solution having phospholipids, ethanol and water, said water comprising 5% (w/w) or less of the phospholipid stock solution;
diluting the phospholipid stock solution with a quantity of ethanol to produce an ethanol-diluted phospholipid stock,
combining said cannabinoid substance with said ethanol-diluted phospholipid stock to produce a cannabinoid-phospholipid stock; and
mixing the cannabinoid-phospholipid stock with a surfactant selected from polysorbates and derivatives, PEG esters of hydrogenated castor oil and derivatives, and combinations thereof, to produce a cannabinoid encapsulate having one or more surfactant-enhanced phospholipid vesicle encapsulating one or more cannabinoid substance therein, said cannabinoid encapsulate having less than 2% water (w/w).
2 . The method of claim 1 , wherein the surfactant is selected from Polysorbate (Tween) 20, Polysorbate (Tween) 80, PEG-40 hydrogenated castor oil, and combinations thereof.
3 . The method of claim 1 , wherein the concentration of cannabinoid substance in said cannabinoid encapsulate is from 3.0 mg/mL to 300 mg/mL.
4 . The method of claim 1 , wherein the phospholipid stock solution has 75%-89% ethanol (w/w), 10%-20% (w/w) phospholipids and 1-5% water (w/w).
5 . The method of claim 1 , wherein cannabinoid encapsulate has up to 25% (w/w) of said surfactant.
6 . The method of claim 1 , wherein said one or more cannabinoid substance further comprises a non-cannabidiol (CBD) substance selected from tetrahydrocannabinol (THC), Cannabis -derived terpenes, Cannabis -derived flavonoids, a whole plant extract of Cannabis sativa , a whole plant extract of Cannabis indica or combinations thereof.
7 . The method of claim 1 , wherein said one or more cannabinoid substance is a Cannabis sativa -derived cannabinoid, Cannabis indica -derived cannabinoid, a synthetically derived cannabinoid, or combinations thereof.
8 . A method of making surfactant-enhanced phospholipid vesicles encapsulating one or more cannabinoid substance comprising the steps of:
providing a cannabinoid substance having one or more cannabinoid compound selected from cannabidiol (CBD), tetrahydrocannabinol (THC), a whole plant extract of Cannabis sativa , a whole plant extract of Cannabis indica , and combinations thereof;
providing a phospholipid stock solution having 10%-20% phospholipids (w/w), 75%-89% (w/w) ethanol and 1%-5% water (w/w),
diluting the phospholipid stock solution with a quantity of ethanol to produce an ethanol-diluted phospholipid stock, and
combining said cannabinoid substance with said ethanol-diluted phospholipid stock to produce a cannabinoid-phospholipid stock; and
mixing the cannabinoid-phospholipid stock with a surfactant in an amount of up to 25% (w/w) to produce a cannabinoid encapsulate having one or more surfactant-enhanced phospholipid vesicle encapsulating said one or more cannabinoid substance, wherein the cannabinoid encapsulate has a cannabinoid concentration of 3.0 mg/mL to 300 mg/mL.
9 . The method of claim 8 , wherein the cannabinoid substance is cannabidiol (CBD).
10 . The method of claim 8 , wherein the cannabinoid substance is tetrahydrocannabinol (THC).
11 . The method of claim 8 , wherein the cannabinoid substance is a combination of cannabidiol (CBD) and tetrahydrocannabinol (THC).
12 . The method of claim 8 , wherein the surfactant is selected from Polysorbates, Polysorbate (Tween) 20, Polysorbate (Tween) 80, PEG esters of hydrogenated castor oil, PEG-40 hydrogenated castor oil, Polyethylene Glycols, Propylene Glycol, Sodium, Potassium or Ammonium Salts of Fatty Acids, and derivatives or combinations thereof.
13 . The method of claim 12 , wherein the surfactant is selected from Polysorbate (Tween) 80, PEG-40 hydrogenated castor oil, and combinations thereof.
14 . The method of claim 8 , wherein the surfactant concentration is 0.5% to 20% (w/w) of the cannabinoid encapsulate.
15 . The method of claim 8 , wherein the cannabinoid encapsulate has less than 2% (w/w) water.
16 . The method of claim 8 , wherein said one or more cannabinoid substance is a Cannabis sativa -derived cannabinoid, Cannabis indica -derived cannabinoid, a synthetically derived cannabinoid, or combinations thereof.
17 . A method of making a surfactant-enhanced phospholipid vesicle encapsulating one or more lipophilic passenger substance comprising the steps of:
providing a lipophilic passenger substance;
providing a phospholipid stock solution having 10%-20% (w/w) phospholipids, 75%-89% (w/w) ethanol and 1%-5% water (w/w);
diluting the phospholipid stock solution with a quantity of ethanol up to 75% (w/w) to produce an ethanol-diluted phospholipid stock,
combining said lipophilic passenger substance with said ethanol-diluted phospholipid stock to produce a passenger-phospholipid stock; and
mixing the passenger-phospholipid stock with a surfactant selected from Polysorbates, Polysorbate (Tween) 20, Polysorbate (Tween) 80, PEG esters of hydrogenated castor oil, PEG-40 hydrogenated castor oil, Polyethylene Glycols, Propylene Glycol, Sodium, Potassium or Ammonium Salts of Fatty Acids, and derivatives or combinations thereof, to produce a passenger encapsulate having one or more surfactant-enhanced phospholipid vesicle encapsulating said one or more lipophilic passenger substance therein, said passenger encapsulate having less than 2% water (w/w).
18 . The method of claim 17 , wherein the lipophilic passenger substance is selected from cannabinoids, cannabidiol, tetrahydrocannabinol, menthol, lidocaine, aspirin, ibuprofen, acetaminophen, antifungals, retinoids, and combinations thereof.
19 . The method of claim 18 , wherein the lipophilic passenger substance is cannabidiol (CBD), tetrahydrocannabinol (THC), or combinations thereof.
20 . The method of claim 17 , wherein the passenger encapsulate has a lipophilic passenger concentration of 3.0 mg/mL to 300 mg/mL.
21 . The method of claim 17 , wherein the surfactant is selected from Polysorbate (Tween) 80, PEG-40 hydrogenated castor oil, and combinations thereof.
22 . The method of claim 17 , wherein the surfactant is 0.5% to 20% (w/w) of the passenger encapsulate.
23 . The method of claim 18 , wherein said one or more cannabinoid substance is a Cannabis sativa -derived cannabinoid, Cannabis indica -derived cannabinoid, a synthetically derived cannabinoid, or combinations thereof.