Compositions comprising bacterial strains
The invention provides compositions comprising bacterial strains for treating and preventing a neurodegenerative disorder.
1. A pharmaceutical composition comprising a lyophilized bacteria strain of the genus Roseburia in a therapeutically effective amount for treating a disorder selected from the group consisting of Parkinson's disease, progressive supranuclear palsy, Steele-Richardson-Olszewski syndrome, normal pressure hydrocephalus, vascular parkinsonism, arteriosclerotic parkinsonism, drug-induced parkinsonism, Alzheimer's disease, Benson's syndrome, Huntington's disease, amyotrophic lateral sclerosis, Lou Gehrig's disease, motor neurone disease, prion disease, spinocerebellar ataxia, spinal muscular atrophy, dementia, Lewy body dementia, vascular dementia, frontotemporal dementia, primary progressive aphasia, mild cognitive impairment, HIV-related cognitive impairment, corticobasal degeneration, and stroke in a subject in need thereof,
wherein said pharmaceutical composition contains only a single bacteria strain of the genus Roseburia and does not contain any other bacterial species or strain, and
wherein said pharmaceutical composition is formulated in an enteric capsule for delivery to an intestine of said subject.
2. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition comprises said bacteria strain in a therapeutically effective amount for treating a disorder selected from the group consisting of an early-onset Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, Lou Gehrig's disease, motor neurone disease, prion disease, spinocerebellar ataxia, spinal muscular atrophy, dementia, primary progressive aphasia, mild cognitive impairment, HIV-related cognitive impairment, and corticobasal degeneration in said subject.
3. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition comprises said bacteria strain in a therapeutically effective amount for delaying an onset or a progression of a disorder selected from the group consisting of Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, Lou Gehrig's disease, motor neurone disease, prion disease, spinocerebellar ataxia, spinal muscular atrophy, dementia, primary progressive aphasia, mild cognitive impairment, HIV-related cognitive impairment, and corticobasal degeneration in said subject.
4. The pharmaceutical composition of claim 1 , wherein said bacteria strain is of species Roseburia hominis , of species Roseburia intestinalis , or of species Roseburia faecis.
5. The pharmaceutical composition of claim 1 , wherein said bacteria strain comprises a 16S rRNA gene sequence that has at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:3, as determined by a Smith Waterman homology search algorithm using an affine gap search with a gap open penalty of 12, a gap extension penalty of 2, and a Blocks Substitution Matrix (BLOSUM) of 62.
6. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition comprises said bacteria strain in a therapeutically effective amount for treating Parkinson's disease in said subject.
7. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition comprises said bacteria strain in a therapeutically effective amount for treating stroke in said subject, and
wherein said stroke is selected from the group consisting of cerebral ischemia, focal cerebral ischemia, ischemic stroke, and hemorrhagic stroke.
8. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition further comprises a pharmaceutically acceptable excipient, diluent, or carrier.
9. The pharmaceutical composition of claim 1 , wherein said bacteria strain comprises a 16S rRNA gene sequence that has at least 97% sequence identity to the polynucleotide sequence of SEQ ID NO:3, as determined by a Smith Waterman homology search algorithm using an affine gap search with a gap open penalty of 12, a gap extension penalty of 2, and a Blocks Substitution Matrix (BLOSUM) of 62.
10. The pharmaceutical composition of claim 1 , wherein said bacteria strain comprises a 16S rRNA gene sequence of SEQ ID NO:3, SEQ ID NO: 5, or SEQ ID NO:6.
11. The pharmaceutical composition of claim 1 , wherein said bacterial strain is the strain deposited under accession number NCIMB 42383 or the strain deposited under accession number NCIMB 43043.
12. The pharmaceutical composition of claim 1 , wherein said bacteria strain is capable of at least partially colonizing an intestine of said subject.