CA IX—NIR dyes and their uses
The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a ligand that binds to the active site of carbonic anhydrase, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid, amide, ureido, or polyethylene glycol derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.
1. A kit comprising:
a first compound having the formula:
B—W—X—Y—Z
wherein B is a CA IX-targeted molecule is selected from the group consisting of
wherein n is 0, 1, 2, or 3; m is 0, 1, 2, 3, 4, or 5 and p is 0, 1, 2, or 3;
W is an extended hydrophobic residue;
X is a hydrophobic spacer;
Y is an amino acid spacer; and
Z is a NIR dye; and
a second compound, wherein the second compound comprises a pteroyl ligand conjugated to an amino acid linking group or a PSMA-targeting compound conjugated to an amino acid linking group.
2. The kit of claim 1 , wherein the kit comprises the second compound and a third compound, wherein the second compound comprises the pteroyl ligand conjugated to the amino acid linking group and the third compound comprises the PSMA-targeting compound conjugated to the amino acid linking group.
3. The kit of claim 1 , wherein Z is selected from the group consisting of LS288, IR800, SP054, S0121, S2076, S0456,
wherein R 41 , R 42 , R 43 , R 44 , R 45 , R 46 , R 47 , R 48 , ═H or SO 3 H, and X=O, S, or N.
4. The kit of claim 3 , wherein Z is S0456.