Methods for sample preparation and testing
A method and system for analyzing a sample potentially comprising a target, comprising: (i) activating one or more components of the sample analysis system in preparation for a sample, wherein the sample analysis system comprises a heating element, a centrifuge, a thermocycler, an imager, a user interface, and a processor; (ii) obtaining a sample potentially comprising a target; (iii) adding the obtained sample to a sample preparation receptacle, the sample preparation receptacle comprising one or more reagents configured to maximize success of the sample analysis; (iv) heating the sample preparation receptacle, with the added sample, in the heating element of the sample analysis system at a first temperature for a first heating period of time; (v) centrifuging the sample preparation receptacle, after heating, for a first period of time using the centrifuge of the sample analysis system; (vi) transferring at least a portion of the sample, after centrifugation, from the sample preparation receptacle to a sample analysis receptacle, the sample analysis receptacle comprising one or more reagents configured for a qPCR reaction specific to a selected target; (vii) obtaining, via the thermocycler and imager of the sample analysis system, a fluorescence curve during a qPCR reaction; (viii) analyzing, by the process of the sample analysis system, the obtained fluorescence curve to determine a presence or absence of the selected target in the sample; and (ix) reporting, to a user by the user interface of the sample analysis system, the presence or absence of the selected target in the sample; wherein one or more steps of the method are guided by interactive instructions provided by the sample analysis system.
1. A method for analyzing a sample using a sample analysis system, comprising:
activating one or more components of the sample analysis system in preparation for a sample of milk, wherein the sample analyses system comprises a heating element, a centrifuge, a thermocycler, an imager, a user interface, and a processor;
obtaining the sample of milk;
adding the sample to a sample preparation receptacle;
heating the sample once in the sample preparation receptacle with the heating element of the sample analysis system at a temperature of 100 degrees Celsius for twenty minutes;
centrifuging the sample preparation receptacle one time, after heating, for a first period of time using the centrifuge of the sample analysis system;
transferring at least a portion of the sample, after centrifugation one time, from the sample preparation receptacle to a sample analysis receptacle, the sample analysis receptacle comprising one or more reagents configured for a quantitative polymerase chain reaction specific to a selected target;
obtaining, via the thermocycler and imager of the sample analysis system, a fluorescence curve during a quantitative polymerase chain reaction;
analyzing, by the processor of the sample analysis system, the obtained fluorescence curve to determine whether the selected target is present in the sample; and
reporting, to a user by the user interface of the sample analysis system, the determination whether the selected target is present in the sample;
wherein the adding, heating, centrifuging and transferring steps are guided by interactive instructions provided to the user via the user interface sample analysis system.
2. The method for analyzing a sample using a sample analysis system of claim 1 , wherein the sample preparation receptacle comprises one or more reagents for the sample analysis.
3. The method for analyzing a sample using a sample analysis system of claim 1 , further comprising the step of transferring a portion of the sample from the sample preparation receptacle to a reagent-containing tube and mixing to remove additional polymerase chain reaction inhibitors.
4. The method for analyzing a sample using a sample analysis system of claim 1 , wherein the instructions comprise a video that demonstrates the action that should be taken by the user.
5. A method for analyzing a sample using a sample analysis system, comprising:
activating one or more components of the sample analysis system in preparation for a sample of milk, wherein the sample analyses system comprises a heating element, a centrifuge, a thermocycler, an imager, a user interface, a processor; and a memory;
obtaining the sample of milk potentially and providing the system with information about the sample of milk which is stored in the memory;
adding the sample of milk to a sample preparation receptacle and providing the system with information about the sample analysis receptacle which is stored in the memory;
heating the sample preparation receptacle one time, with the added sample, in the heating element of the sample analysis system at a first temperature of 100 degrees Celsius for twenty minutes;
centrifuging the sample preparation receptacle one time, after heating one time, for a first period of time using the centrifuge of the sample analysis system;
transferring at least a portion of the sample, after centrifugation one time, from the sample preparation receptacle to a sample analysis receptacle, the sample analysis receptacle comprising one or more reagents configured for a quantitative polymerase chain reaction specific to a selected target, and providing the system with information about the sample analysis receptacle which is stored in the memory;
cycling via thermocycler, wherein the identification of the sample analysis receptacle is recalled from the memory and used to determine directions for the polymerase chain reaction program;
obtaining, via the thermocycler and imager of the sample analysis system, a fluorescence curve during a quantitative polymerase chain reaction;
analyzing, by the processor of the sample analysis system, the obtained fluorescence curve to determine whether the selected target is present in the sample; and
reporting, to a user by the user interface of the sample analysis system, whether the selected target is present in the sample;
wherein the adding, heating, centrifuging and transferring steps are guided by interactive instructions provided to the user via the user interface sample analysis system.
6. The method for analyzing a sample using a sample analysis system of claim 5 , wherein the sample preparation receptacle comprises one or more reagents for the sample analysis.
7. The method for analyzing a sample using a sample analysis system of claim 5 , further comprising the step of transferring a portion of the sample from the sample preparation receptacle to a reagent-containing tube and mixing to remove additional polymerase chain reaction inhibitors.
8. The method for analyzing a sample using a sample analysis system of claim 5 , wherein the instructions comprise a video that demonstrates the action that should be taken by the user.
9. The method for analyzing a sample using a sample analysis system of claim 5 , wherein information about the sample preparation receptacle and the sample analysis receptacle is provided system via a scanner.
10. The method for analyzing a sample using a sample analysis system of claim 9 , wherein the sample preparation receptacle comprises a barcode containing information about the receptacle.