Granted Patent
B2
US 12,383,656 · App. 16/728,863 · Granted Aug 12, 2025
Tissue engineered intestine
A61L27/3882A61L27/18A61L27/227A61L27/38A61L27/3826A61L27/383A61L27/3873A61L27/3886A61L27/3891A61L27/3895A61L27/56A61L2400/12A61L2430/22
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Abstract
The invention provides for engineered intestinal construct and methods of making these constructs. The invention also provides for methods of treating short bowel syndrome or methods of repairing an intestine after resection comprising inserting an engineered intestinal construct into the intestine of a subject in need.
Claims (11)
1. An engineered intestine construct comprising a nanofiber scaffold seeded with neural stem cells, smooth muscle cells and intestinal stem cells from intestinal crypts, and wherein the nanofiber scaffold comprises HB-EGF polypeptide or a fragment thereof.
2. An engineered intestine construct comprising a nanofiber scaffold seeded with neural stem cells, smooth muscle cells and intestinal stem cells from intestinal crypts, wherein at least one of the neural stem cells, smooth muscle cells or intestinal stem cells overexpress HB-EGF polypeptide or a fragment thereof.
3. An engineered intestine construct comprising a multilayer nanofiber scaffold,
wherein the multilayer nanofiber scaffold comprises at least an inner layer and an outer layer,
wherein the outer layer comprises neural stem cells and smooth muscle cells, and
wherein the inner layer comprises intestinal stem cells.
4. The engineered intestine construct of claim 3 wherein at least one of the layers comprises HB-EGF polypeptide or a fragment.
5. The engineered intestine construct of claim 3 wherein the construct further comprises at least one middle layer.
6. The engineered intestine construct of claim 1 wherein the nanofiber scaffold comprises Poly (glycolic acid) (PGA) nanofibers, Poly (ε-caprolactone) (PCL) nanofibers, Poly (-caprolactone-co-lactic acid) (PLC) nanofibers, Poly (L-lactic acid) (PLLA) nanofibers, Poly (D-lactic acid-co-glycolic acid) (PDLGA) nanofibers, Poly (D-lactic acid-co-glycolic acid) (PLGA) nanofibers, Polyurethane (PU) nanofibers, Polydioxanone (PDO) nanofibers or a combination thereof.
7. The engineered intestine construct of claim 1 wherein the construct comprises a layer of macrofibers between two layers.
8. The engineered intestine construct of claim 7 where the layer of macrofibers comprises PGA.
Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 9, 2025
From: BESNER, GAIL E.; LIU, YANCHUN E.
To: RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL
Reel/Frame 071646/0929 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 9, 2025
From: JOHNSON, JED
To: NANOFIBER SOLUTIONS LLC
Reel/Frame 071647/0414 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 4, 2022
From: NANOFIBER SOLUTIONS, LLC
To: NFS IP HOLDINGS, LLC
Reel/Frame 061882/0663 →
Continuity (3)
Continuation
14774045
Provisional Application
61783655
· Mar 14, 2013
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