Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof
The present invention provides compositions and methods based on genetic polymorphisms that are associated with cardiovascular diseases, particularly coronary heart disease (especially myocardial infarction) or hypertension. For example, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by these nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and variant proteins, and methods of using the nucleic acid molecules and proteins as well as methods of using reagents for their detection.
1. A method for reducing risk of myocardial infarction in a human, the method comprising:
a) receiving an identification of a human as having an increased risk for myocardial infarction due to a polymorphism rs2954029 comprising A at position 101 of SEQ ID NO:3944 or T at its complement; and
b) administering a statin to said human.
2. The method of claim 1 , wherein said human is homozygous for said A or said T.
3. The method of claim 1 , wherein said human is heterozygous for said A or said T.
4. The method of claim 1 , wherein said myocardial infarction is recurrent myocardial infarction.
5. A method for identifying a human as having an increased risk for myocardial infarction, the method comprising:
a) testing nucleic acid from said human for a polymorphism rs2954029 as represented by position 101 of SEQ ID NO:3944 or its complement by contacting said nucleic acid with an oligonucleotide consisting of a segment of SEQ ID NO:3944 or an oligonucleotide consisting of a segment of the complement of SEQ ID NO:3944, wherein said oligonucleotide specifically hybridizes to A at position 101 of SEQ ID NO:3944 or T at position 101 of the complement of SEQ ID NO:3944;
b) detecting said A or said T; and
c) identifying said human as having an increased risk for myocardial infarction due to the presence of said A or said T.
6. The method of claim 5 , wherein said method comprises preparing a sample from said human that is enriched for a fragment of said nucleic acid that includes said position 101 of SEQ ID NO:3944 or its complement by amplifying said fragment by polymerase chain reaction (PCR).
7. The method of claim 5 , wherein said human is homozygous for said A or said T.
8. The method of claim 5 , wherein said human is heterozygous for said A or said T.
9. The method of claim 5 , wherein said oligonucleotide is detectably labeled with a fluorescent dye.
10. The method of claim 5 , wherein said testing comprises allele-specific amplification, and further wherein said detecting comprises detecting the presence of an amplicon.
11. The method of claim 5 , wherein said myocardial infarction is recurrent myocardial infarction.
12. A method for identifying a human as having an increased risk for myocardial infarction, the method comprising:
a) amplifying by polymerase chain reaction (PCR) a fragment of nucleic acid from said human that includes a polymorphism rs2954029 as represented by position 101 of SEQ ID NO:3944 or its complement to thereby create an amplicon containing said polymorphism;
b) testing for said polymorphism by contacting said amplicon with an oligonucleotide consisting of a segment of SEQ ID NO:3944 or an oligonucleotide consisting of a segment of the complement of SEQ ID NO:3944, wherein said oligonucleotide specifically hybridizes to A at position 101 of SEQ ID NO:3944 or T at position 101 of the complement of SEQ ID NO:3944;
c) detecting said A or said T; and
d) identifying said human as having an increased risk for myocardial infarction due to the presence of said A or said T.
13. The method of claim 12 , wherein said human is homozygous for said A or said T.
14. The method of claim 12 , wherein said human is heterozygous for said A or said T.
15. The method of claim 12 , wherein said oligonucleotide is detectably labeled with a fluorescent dye.
16. The method of claim 12 , wherein said myocardial infarction is recurrent myocardial infarction.