Method of sample preparation on a spectrometric sample support
View Patent ↗The invention relates to a method for sample preparation on a spectrometric sample support, comprising the steps of: (i) providing the sample support with an arrangement of individual liquid droplets, for example of washing liquid or nutrient solution, each of which has microorganism sediments enclosed in it; (ii) locating a plate of an absorbent material containing cotton fibers, for example, above the sample support; (iii) lowering the plate vertically onto the sample support in such a way that microorganism sediments and plate come into contact, whereby the droplets of liquid are absorbed into the absorbent material; (iv) lifting the plate, which is locally enriched with droplet liquid, off the sample support, thereby exposing the microorganism sediments that are depleted of liquid; and (v) preparing the exposed microorganism sediments for spectrometric measurement, by means of infrared spectrometry or MALDI time-of-flight mass spectrometry, for example.
1. A method for sample preparation on a spectrometric sample support, comprising the steps of:
providing an arrangement of individual droplets of liquid on the sample support, each of which has microorganism sediments enclosed in it;
locating a plate of absorbent material above the sample support;
lowering the plate vertically onto the sample support in such a way that the microorganism sediments and plate come into contact, whereby the droplets of liquid are absorbed into the absorbent material;
lifting the plate off the sample support, thereby exposing the microorganism sediments that are depleted of liquid; and
preparing the exposed microorganism sediments for spectrometric measurement.
2. The method according to claim 1 , wherein the plate contains a swelling absorbent material and is lowered until there is a small enough gap between it and the sample support that a supernatant of the droplets comes into contact with the plate and is absorbed by it, whereby the absorbent material undergoes localized swelling at the sites where the droplet liquid is absorbed and makes contact with the microorganism sediments.
3. The method according to claim 1 , wherein the plate is placed directly onto the sample support and pressed against it so that the entire arrangement of droplets makes contact with the plate.
4. The method according to claim 3 , wherein the sample support and/or the plate are spring-mounted with relative respect to each other so that pressure applied is taken up by springs when the plate is pressed down.
5. The method according to claim 1 , further comprising moistening a plate surface facing the sample support before said step of lowering.
6. The method according to claim 1 , wherein the plate comprises absorbent fibers of cellulose and/or plant fibers.
7. The method according to claim 1 , wherein a volume of one of said droplets is approximately one to twelve microliters.
8. The method according to claim 1 , wherein the liquid droplets comprise nutrient solution or washing liquid.
9. The method according to claim 8 , wherein the liquid droplets further comprise an antimicrobial substance.
10. The method according to claim 1 , further comprising performing an infrared or mass spectrometric measurement after preparing the exposed microorganism sediments.
11. The method according to claim 1 , wherein any remaining residual liquid film on the microorganism cell sediments is allowed to dry after lifting the plate before preparing the exposed microorganism sediments.