Methods and compositions for diagnosing and treating diseases
Methods and compositions are provided for assessing, treating, and preventing diseases, especially cancer, using cancer-associated targets (CAT). Methods and compositions are also provided for determining or predicting the effectiveness of a treatment for these diseases or for selecting a treatment, using CAT. Methods and compositions are further provided for modulating cell function using CAT. Also provided are compositions that modulate CAT (e.g., antagonists or agonists), such as antibodies, proteins, small molecule compounds, and nucleic acid agents (e.g., RNAi and antisense agents), as well as pharmaceutical compositions thereof. Further provided are methods of screening for agents that modulate CAT, and agents identified by these screening methods.
1. A method of inhibiting proliferation of pancreatic cancer cells, the method comprising contacting the pancreatic cancer cells with an oligonucleotide that selectively hybridizes to a SLC5A6 mRNA molecule thereby inhibiting proliferation of the pancreatic cancer cells, wherein the sequence of the oligonucleotide comprises a sequence selected from the group consisting of:
nucleotides 659-677 of SEQ ID NO:77,
nucleotides 1185-1203 of SEQ ID NO:77,
nucleotides 2059-2077 of SEQ ID NO:77, and
nucleotides 2161-2179 of SEQ ID NO:77.
2. The method of claim 1 , wherein the inhibiting proliferation comprises RNA interference (RNAi).
3. The method of claim 1 , wherein the oligonucleotide is an siRNA or antisense oligonucleotide.
4. The method of claim 1 , wherein the contacting comprises administering a therapeutically effective amount of the oligonucleotide to an individual harboring the pancreatic cancer cells.
5. The method of claim 1 , wherein the method is performed in vitro.
6. The method of claim 1 , wherein the nucleic acid sequence of the SLC5A6 mRNA molecule comprises a sequence selected from the group consisting of SEQ ID NOS:10-12 and 77.
7. The method of claim 1 , wherein the nucleic acid sequence of the SLC5A6 mRNA molecule comprises SEQ ID NO:77.
8. A method of inhibiting proliferation of pancreatic cancer cells, the method comprising contacting the pancreatic cancer cells with an oligonucleotide that selectively hybridizes to a SLC5A6 mRNA molecule thereby inhibiting proliferation of the pancreatic cancer cells, wherein the sequence of the oligonucleotide consists of a sequence selected from the group consisting of:
nucleotides 659-677 of SEQ ID NO:77,
nucleotides 1185-1203 of SEQ ID NO:77,
nucleotides 2059-2077 of SEQ ID NO:77, and
nucleotides 2161-2179 of SEQ ID NO:77.
9. The method of claim 8 , wherein the inhibiting proliferation comprises RNA interference (RNAi).
10. The method of claim 8 , wherein the oligonucleotide is an siRNA or antisense oligonucleotide.
11. The method of claim 8 , wherein the contacting comprises administering a therapeutically effective amount of the oligonucleotide to an individual harboring the pancreatic cancer cells.
12. The method of claim 8 , wherein the method is performed in vitro.
13. The method of claim 8 , wherein the nucleic acid sequence of the SLC5A6 mRNA molecule comprises a sequence selected from the group consisting of SEQ ID NOS: 10-12 and 77.
14. The method of claim 8 , wherein the nucleic acid sequence of the SLC5A6 mRNA molecule comprises SEQ ID NO:77.