Multimeric IL-15 soluble fusion molecules and methods of making and using same
The present invention features compositions and methods featuring ALT-803, a complex of an interleukin-15 (IL-15) superagonist mutant and a dimeric IL-15 receptor α/Fc fusion protein useful for enhancing an immune response against a neoplasia (e.g., multiple myeloma, melanoma, lymphoma) or a viral infection (e.g., human immunodeficiency virus).
1. A kit comprising: an effective amount of an interleukin-15N72D:IL-15 receptor alpha Sushi/Fc (IL-15N72D:IL-15RαSu/Fc) complex, wherein the complex comprises a dimeric IL-15RαSu/Fc and two IL-15N72D molecules (ALT-803); and directions for use of the kit.
2. The kit of claim 1 wherein the IL-15RαSu/Fc comprises SEQ ID NO.: 1.
3. The kit of claim 1 , wherein a IL-15N72D molecule comprises SEQ ID NO.: 2.
4. The kit of claim 1 , wherein the effective amount is between about 1 and 20 μg/kg.
5. The kit of claim 1 , wherein the effective amount is about 1 μg/kg.
6. The kit of claim 1 , wherein the effective amount is about 5 μg/kg.
7. The kit of claim 1 , wherein the effective amount is about 10 μg/kg.
8. The kit of claim 1 , wherein the effective amount is about 20 μg/kg.
9. The kit of claim 1 , wherein the effective amount is between about 20 μg and 100 μg/kg.
10. The kit of claim 1 , wherein the effective amount is about 30 μg/kg.
11. The kit of claim 1 , wherein the effective amount is about 50 μg/kg.
12. The kit of claim 1 , wherein the effective amount is about 75 μg/kg.
13. The kit of claim 1 , wherein the effective amount is about 100 μg/kg.