Granted Patent
B2
US 12,378,543 · App. 16/756,803 · Granted Aug 5, 2025
Compositions for hemolysis and coagulation regulation and stabilization of extracellular vesicles
Assignee:
STRECK LLC
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Abstract
A composition and method of use thereof wherein the composition comprises one or more components capable of releasing an aldehyde, one or more anticoagulants or chelating agents, and one or more polysaccharides. The composition has a pH of from about 4 to about 6.
Claims (21)
1. A composition comprising:
(i) a component capable of releasing an aldehyde;
(ii) an anticoagulant comprising citric acid, trisodium citrate, and dextrose; and
(iii) α-cyclodextrin,
wherein the component capable of releasing an aldehyde is imidazolidinyl urea.
2. The composition of claim 1 , wherein the anticoagulant is anticoagulant citrate dextrose-A (ACD-A), anticoagulant citrate dextrose-B (ACD-B), or citrate-phosphate-dextrose-adenine (CPDA).
3. The composition of claim 1 , wherein the dextrose is present at a concentration of from about 2% to about 20% (weight/volume).
4. The composition of claim 3 , wherein citrate ion concentration is from about 200 mM to about 500 mM.
5. The composition of claim 1 , wherein the citric acid is present at a concentration of from about 0.5% to about 4% (weight/volume).
6. The composition of claim 5 , wherein the trisodium citrate is present at a concentration of from about 3% to about 15% (weight/volume).
7. The composition of claim 1 , wherein the component capable of releasing an aldehyde is present at a concentration of from about 10% to about 40% (weight/volume).
8. The composition of claim 1 , wherein the α-cyclodextrin is present at a concentration of from about 0.75% to about 4%.
9. A method of treating a single blood sample with the composition of claim 1 , comprising contacting the sample with the composition and isolating one or more of DNA, RNA, extracellular vesicles, circulating tumor cells, circulating rare cells, or proteins from the sample.
10. A method of inhibiting lysis of a cell comprising contacting the cell with a composition of claim 1 , wherein the inhibiting prevents release of nucleic acid and/or an extracellular vesicle from the cell.
11. The method of claim 10 , wherein the cell is a white blood cell.
12. The method of claim 10 , wherein the cell is a red blood cell.
13. The method of claim 10 , wherein the nucleic acid is cell free RNA (cfRNA), cell free DNA (cfDNA), cellular RNA, or cellular DNA.
14. The method of claim 10 , wherein lysis is inhibited for at least about 24 hours.
15. The method of claim 14 , wherein lysis is inhibited by at least 2 days, or at least 3 days, or at least 4 days.
16. The method of claim 10 , further comprising maintaining the cell for at least about 24 hours and then isolating the nucleic acid from the cell.
17. The method of claim 16 , wherein the cell is maintained at room temperature.
Assignments (4)
SECURITY INTEREST
Recorded Feb 21, 2023
From: STRECK LLC
To: JPMORGAN CHASE BANK, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 062819/0851 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 16, 2023
From: STRECK, INC.
To: STRECK LLC
Reel/Frame 062766/0015 →
SECURITY INTEREST
Recorded Apr 23, 2021
From: STRECK, INC.
To: JPMORGAN CHASE BANK, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 056016/0124 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 12, 2020
From: GEORGE, NICHOLAS MICHAEL; HUNSLEY, BRADFORD A.
To: STRECK, INC.
Reel/Frame 053466/0452 →
Continuity (2)
Provisional Application
62574515
· Oct 19, 2017
References Cited (400)
US 1432249A
· Hoyme
· 1922
[cited by applicant]
US 1922799A
· Gaus
· 1933
[cited by applicant]
US 2250666A
· Webb
· 1941
[cited by applicant]
US 2690624A
· Phillips
· 1954
[cited by applicant]
US 2930570A
· Leedy
· 1960
[cited by applicant]
US 3781120A
· Engelhardt
· 1973
[cited by applicant]
US 3867521A
· Miskel et al.
· 1975
[cited by applicant]
US 3872730A
· Ringrose et al.
· 1975
[cited by applicant]
US 3874384A
· Deindoerfer et al.
· 1975
[cited by applicant]
US 3879295A
· Glover et al.
· 1975
[cited by applicant]
US 3973913A
· Louderback
· 1976
[cited by applicant]
US 3994085A
· Groselak et al.
· 1976
[cited by applicant]
US 4043453A
· Greenlee
· 1977
[cited by applicant]
US 4318090A
· Narlow et al.
· 1982
[cited by applicant]
US 4436821A
· Ryan
· 1984
[cited by applicant]
US 4513522A
· Selenke
· 1985
[cited by applicant]
US 4515890A
· Manderino et al.
· 1985
[cited by applicant]
US 4579759A
· Breuers
· 1986
[cited by applicant]
US 4584219A
· Baartmans
· 1986
[cited by applicant]
US 4675159A
· Al-Sioufi
· 1987
[cited by applicant]
US 4818700A
· Cregg et al.
· 1989
[cited by applicant]
US 4884827A
· Kelley
· 1989
[cited by applicant]
US 4921277A
· McDonough
· 1990
[cited by applicant]
US 5000484A
· Phelan et al.
· 1991
[cited by applicant]
US 5060672A
· Irimi et al.
· 1991
[cited by applicant]
US 5110908A
· Deich et al.
· 1992
[cited by applicant]
US 5135125A
· Andel et al.
· 1992
[cited by applicant]
US 5196182A
· Ryan
· 1993
[cited by applicant]
US 5213765A
· Kasai et al.
· 1993
[cited by applicant]
US 5250438A
· Ryan
· 1993
[cited by applicant]
US 5257633A
· Vogler et al.
· 1993
[cited by applicant]
US 5260048A
· Ryan
· 1993
[cited by applicant]
US 5270208A
· Ryan
· 1993
[cited by applicant]
US 5343647A
· Bulka
· 1994
[cited by applicant]
US 5366249A
· Diemert
· 1994
[cited by applicant]
US 5429797A
· Camiener
· 1995
[cited by applicant]
US 5447842A
· Simons
· 1995
[cited by applicant]
US 5457024A
· Goldbard
· 1995
[cited by applicant]
US 5459073A
· Ryan
· 1995
[cited by applicant]
US 5459253A
· Wolin et al.
· 1995
[cited by applicant]
US 5460797A
· Ryan
· 1995
[cited by applicant]
US 5468022A
· Linder et al.
· 1995
[cited by applicant]
US 5490658A
· Coward et al.
· 1996
[cited by applicant]
US 5501954A
· Mahr et al.
· 1996
[cited by applicant]
US 5501963A
· Burckhardt
· 1996
[cited by applicant]
US 5512343A
· Shaw
· 1996
[cited by applicant]
US 5538871A
· Nuovo et al.
· 1996
[cited by applicant]
US 5540358A
· Wiles et al.
· 1996
[cited by applicant]
US 5560657A
· Morgan
· 1996
[cited by applicant]
US 5614391A
· Franciskovich et al.
· 1997
[cited by applicant]
US 5618664A
· Kiessling
· 1997
[cited by applicant]
US 5629147A
· Asgari et al.
· 1997
[cited by applicant]
US D382343S
· Wandell et al.
· 1997
[cited by applicant]
US 5654054A
· Tropsha et al.
· 1997
[cited by applicant]
US 5688516A
· Raad et al.
· 1997
[cited by applicant]
US 5731156A
· Golbus
· 1998
[cited by applicant]
US 5741638A
· Yamane
· 1998
[cited by applicant]
US 5783093A
· Holme
· 1998
[cited by applicant]
US 5811099A
· Ryan
· 1998
[cited by applicant]
US 5817519A
· Zelmanovic et al.
· 1998
[cited by applicant]
US 5849517A
· Ryan
· 1998
[cited by applicant]
US 5858699A
· Granger et al.
· 1999
[cited by applicant]
US 5861253A
· Asgari et al.
· 1999
[cited by applicant]
US 5888822A
· Hengstenberg
· 1999
[cited by applicant]
US 5906744A
· Carroll et al.
· 1999
[cited by applicant]
US 5939259A
· Harvey et al.
· 1999
[cited by applicant]
US 5962234A
· Golbus
· 1999
[cited by applicant]
US 5976014A
· Petrick et al.
· 1999
[cited by applicant]
US 5977153A
· Camiener
· 1999
[cited by applicant]
US 5985572A
· MacFarlane
· 1999
[cited by applicant]
US 6013240A
· Behr et al.
· 2000
[cited by applicant]
US 6030767A
· Wagner et al.
· 2000
[cited by applicant]
US 6043032A
· Yamagishi
· 2000
[cited by applicant]
US 6072086A
· James et al.
· 2000
[cited by applicant]
US 6074825A
· Rundell et al.
· 2000
[cited by applicant]
US 6074827A
· Nelson et al.
· 2000
[cited by applicant]
US 6077235A
· Serpentino et al.
· 2000
[cited by applicant]
US 6125563A
· Girerd
· 2000
[cited by applicant]
US 6128840A
· Boisvert
· 2000
[cited by applicant]
US 6168922B1
· Harvey et al.
· 2001
[cited by applicant]
US 6177163B1
· Blok et al.
· 2001
[cited by applicant]
US 6190609B1
· Chapman et al.
· 2001
[cited by applicant]
US 6197539B1
· Granger et al.
· 2001
[cited by applicant]
US 6197540B1
· Granger et al.
· 2001
[cited by applicant]
US 6200500B1
· Ryan
· 2001
[cited by applicant]
US 6210889B1
· Drouin et al.
· 2001
[cited by applicant]
US 6218531B1
· Ekenberg
· 2001
[cited by applicant]
US 6221668B1
· Ryan et al.
· 2001
[cited by applicant]
US 6251638B1
· Umansky et al.
· 2001
[cited by applicant]
US 6258540B1
· Lo et al.
· 2001
[cited by applicant]
US 6287820B1
· Umansky et al.
· 2001
[cited by applicant]
US 6337189B1
· Ryan
· 2002
[cited by applicant]
US 6365362B1
· Terstappen et al.
· 2002
[cited by applicant]
US 6399388B1
· Ryan et al.
· 2002
[cited by applicant]
US 6403377B1
· Ryan et al.
· 2002
[cited by applicant]
US 6406915B2
· Ryan et al.
· 2002
[cited by applicant]
US 6527242B1
· Kennedy
· 2003
[cited by applicant]
US 6527957B1
· Deniega et al.
· 2003
[cited by applicant]
US 6551267B1
· Cohen et al.
· 2003
[cited by applicant]
US 6560847B2
· Ohlsson
· 2003
[cited by applicant]
US 6579672B1
· Granger et al.
· 2003
[cited by applicant]
US 6581973B2
· Levine et al.
· 2003
[cited by applicant]
US 6602718B1
· Augello et al.
· 2003
[cited by applicant]
US 6617170B2
· Augello et al.
· 2003
[cited by applicant]
US 6617180B1
· Wang
· 2003
[cited by applicant]
US 6623983B1
· Terstappen et al.
· 2003
[cited by applicant]
US 6630301B1
· Gocke et al.
· 2003
[cited by applicant]
US 6645731B2
· Terstappen et al.
· 2003
[cited by applicant]
US 6664056B2
· Lo et al.
· 2003
[cited by applicant]
US 6759217B2
· Kopreski
· 2004
[cited by applicant]
US 6821789B2
· Augello et al.
· 2004
[cited by applicant]
US 6860513B2
· Kaufman
· 2005
[cited by applicant]
US 6884573B2
· Fischer et al.
· 2005
[cited by applicant]
US 6913932B2
· Maples et al.
· 2005
[cited by applicant]
US 6916634B2
· Kopreski
· 2005
[cited by applicant]
US 6939671B2
· Kopreski
· 2005
[cited by applicant]
US 6994790B2
· Corbin et al.
· 2006
[cited by applicant]
US 7022478B2
· Rainer et al.
· 2006
[cited by applicant]
US 7044941B2
· Mathias et al.
· 2006
[cited by applicant]
US 7208275B2
· Gocke et al.
· 2007
[cited by applicant]
US 7267980B1
· Mortari et al.
· 2007
[cited by applicant]
US 7282371B2
· Helftenbein
· 2007
[cited by applicant]
US 7288380B1
· Gocke et al.
· 2007
[cited by applicant]
US 7318293B2
· Ardern, II
· 2008
[cited by applicant]
US 7332277B2
· Dhallan
· 2008
[cited by applicant]
US 7332288B2
· Terstappen et al.
· 2008
[cited by applicant]
US 7358039B2
· Fischer et al.
· 2008
[cited by applicant]
US 7390663B2
· Ryan et al.
· 2008
[cited by applicant]
US 7398999B2
· Kaufman
· 2008
[cited by applicant]
US 7419832B2
· Hunsley et al.
· 2008
[cited by applicant]
US 7442506B2
· Dhallan
· 2008
[cited by applicant]
US 7445901B2
· Kudlicki et al.
· 2008
[cited by applicant]
US 7478513B2
· Baldwin
· 2009
[cited by applicant]
US 7569350B2
· Gocke et al.
· 2009
[cited by applicant]
US 7651838B2
· Paterlini-Brechot
· 2010
[cited by applicant]
US 7727720B2
· Dhallan
· 2010
[cited by applicant]
US 7767460B2
· Hunsley et al.
· 2010
[cited by applicant]
US 8304187B2
· Fernando
· 2012
[cited by applicant]
US 8546144B2
· Das et al.
· 2013
[cited by applicant]
US 8551784B2
· Das et al.
· 2013
[cited by applicant]
US 8586306B2
· Fernando
· 2013
[cited by applicant]
US 8841077B2
· Paige et al.
· 2014
[cited by applicant]
US 9034635B2
· Termaat et al.
· 2015
[cited by applicant]
US 9040255B2
· Tsinberg et al.
· 2015
[cited by applicant]
US 9120840B2
· Janssen et al.
· 2015
[cited by applicant]
US 9120849B2
· Chiklis et al.
· 2015
[cited by applicant]
US 9127048B2
· Chiklis et al.
· 2015
[cited by applicant]
US 9127049B2
· MacAgno et al.
· 2015
[cited by applicant]
US 9657227B2
· Fernando
· 2017
[cited by applicant]
US 9926590B2
· Fernando
· 2018
[cited by applicant]
US 9926950B2
· Ooki et al.
· 2018
[cited by applicant]
US 9956281B2
· Ryan et al.
· 2018
[cited by applicant]
US 20010018192A1
· Terstappen et al.
· 2001
[cited by applicant]
US 20010049895A1
· Burke
· 2001
[cited by applicant]
US 20010051341A1
· Lo et al.
· 2001
[cited by applicant]
US 20020045196A1
· Mahoney et al.
· 2002
[cited by applicant]
US 20020066216A1
· Delacruz
· 2002
[cited by applicant]
US 20020086346A1
· Ryan
· 2002
[cited by applicant]
US 20020119503A1
· Ryan et al.
· 2002
[cited by applicant]
US 20030232377A1
· Thomas
· 2003
[cited by applicant]
US 20040014107A1
· Garcia-Blanco et al.
· 2004
[cited by applicant]
US 20050029559A9
· Ahn et al.
· 2005
[cited by applicant]
US 20050107316A1
· Strebhardt et al.
· 2005
[cited by applicant]
US 20050164241A1
· Hahn et al.
· 2005
[cited by applicant]
US 20050181353A1
· Rao et al.
· 2005
[cited by applicant]
US 20050277204A1
· Hollis et al.
· 2005
[cited by applicant]
US 20060008807A1
· O'Hara et al.
· 2006
[cited by applicant]
US 20060105372A1
· Bair et al.
· 2006
[cited by applicant]
US 20060194192A1
· Rao et al.
· 2006
[cited by applicant]
US 20070111233A1
· Bianchi et al.
· 2007
[cited by applicant]
US 20070178478A1
· Dhallan et al.
· 2007
[cited by applicant]
US 20070243548A1
· Georges et al.
· 2007
[cited by applicant]
US 20070243549A1
· Bischoff
· 2007
[cited by applicant]
US 20080081689A1
· Seelig et al.
· 2008
[cited by applicant]
US 20080096217A1
· Kopreski
· 2008
[cited by applicant]
US 20080108071A1
· Thompson
· 2008
[cited by applicant]
US 20080119645A1
· Griffey et al.
· 2008
[cited by applicant]
US 20080206866A1
· Zieglschmid et al.
· 2008
[cited by applicant]
US 20080261292A1
· Kopreski
· 2008
[cited by applicant]
US 20080318801A1
· Leung
· 2008
[cited by applicant]
US 20090011043A1
· Xie
· 2009
[cited by applicant]
US 20090081678A1
· Ryan et al.
· 2009
[cited by applicant]
US 20090197275A1
· Boonyarantanakornkit et al.
· 2009
[cited by applicant]
US 20090215036A1
· Stropp et al.
· 2009
[cited by applicant]
US 20090308303A1
· Burlando
· 2009
[cited by applicant]
US 20100167271A1
· Ryan
· 2010
[cited by applicant]
US 20100216153A1
· Lapidus et al.
· 2010
[cited by applicant]
US 20110027771A1
· Deng
· 2011
[cited by examiner]
US 20110053208A1
· Reiss et al.
· 2011
[cited by applicant]
US 20110110975A1
· Grunkemeyer et al.
· 2011
[cited by applicant]
US 20110111410A1
· Ryan et al.
· 2011
[cited by applicant]
US 20110275536A1
· Kas
· 2011
[cited by applicant]
US 20130209985A1
· Dudaronek et al.
· 2013
[cited by applicant]
US 20140080112A1
· Ryan et al.
· 2014
[cited by applicant]
US 20140199681A1
· Ryan et al.
· 2014
[cited by applicant]
US 20150030578A1
· Releford et al.
· 2015
[cited by applicant]
US 20150301037A1
· Tsinberg et al.
· 2015
[cited by applicant]
US 20160143268A1
· Ryan
· 2016
[cited by applicant]
US 20170052173A1
· Hunsley et al.
· 2017
[cited by applicant]
US 20170097361A1
· Alt et al.
· 2017
[cited by applicant]
US 20170145475A1
· Hunsley
· 2017
[cited by examiner]
US 20180243406A1
· Ryan et al.
· 2018
[cited by applicant]
US 20190177774A1
· Connelly et al.
· 2019
[cited by applicant]
AU 2008288601A1
· 2009
[cited by applicant]
CA 2406463A1
· 2001
[cited by applicant]
CN 1665554A
· 2005
[cited by applicant]
CN 101148658A
· 2008
[cited by applicant]
CN 104070871A
· 2014
[cited by applicant]
CN 104381245A
· 2015
[cited by applicant]
CN 104634628A
· 2015
[cited by applicant]
CN 105985904A
· 2016
[cited by applicant]
CN 107525818A
· 2017
[cited by applicant]
DE 19928820A1
· 2000
[cited by applicant]
EP 1031626A1
· 2000
[cited by applicant]
EP 1207208A2
· 2002
[cited by applicant]
EP 1212613A1
· 2002
[cited by applicant]
EP 1217372A1
· 2002
[cited by applicant]
EP 1425294A2
· 2004
[cited by applicant]
EP 1816461A1
· 2007
[cited by applicant]
EP 1889921A2
· 2008
[cited by applicant]
EP 2216416A1
· 2010
[cited by applicant]
EP 2228453A1
· 2010
[cited by applicant]
EP 2411808A2
· 2012
[cited by applicant]
EP 2674502A1
· 2013
[cited by applicant]
EP 2704740A2
· 2014
[cited by applicant]
EP 2814981A2
· 2014
[cited by applicant]
EP 2832346A1
· 2015
[cited by applicant]
EP 3118623A1
· 2017
[cited by applicant]
EP 3225699A1
· 2017
[cited by applicant]
EP 3572531A1
· 2019
[cited by applicant]
EP 3662080A1
· 2020
[cited by applicant]
JP 2003344389A
· 2003
[cited by applicant]
JP 4453999B2
· 2010
[cited by applicant]
JP 2014012656A
· 2014
[cited by applicant]
JP 2017058326A
· 2017
[cited by applicant]
WO 9010715A1
· 1990
[cited by applicant]
WO 9305650A1
· 1993
[cited by applicant]
WO 9402646A1
· 1994
[cited by applicant]
WO 9526417A1
· 1995
[cited by applicant]
WO 9745729A1
· 1997
[cited by applicant]
WO 9802528A1
· 1998
[cited by applicant]
WO 9802740A1
· 1998
[cited by applicant]
WO 9844158A1
· 1998
[cited by applicant]
WO 9859042A1
· 1998
[cited by applicant]
WO 9906594A1
· 1999
[cited by applicant]
WO 0000813A1
· 2000
[cited by applicant]
WO 0006780A1
· 2000
[cited by applicant]
WO 0075647A1
· 2000
[cited by applicant]
WO 0077235A1
· 2000
[cited by applicant]
WO 0114872A1
· 2001
[cited by applicant]
WO 0179851A1
· 2001
[cited by applicant]
WO 0198542A2
· 2001
[cited by applicant]
WO 0234952A2
· 2002
[cited by applicant]
WO 0255985A2
· 2002
[cited by applicant]
WO 0256030A2
· 2002
[cited by applicant]
WO 0318757A2
· 2003
[cited by applicant]
WO 0319141A2
· 2003
[cited by applicant]
WO 0335895A2
· 2003
[cited by applicant]
WO 0369344A1
· 2003
[cited by applicant]
WO 0374730A1
· 2003
[cited by applicant]
WO 2003074723A2
· 2003
[cited by applicant]
WO 0395974A2
· 2003
[cited by applicant]
WO 2003094990A1
· 2003
[cited by applicant]
WO 2006100063A2
· 2006
[cited by applicant]
WO 2007022483A2
· 2007
[cited by applicant]
WO 2008107724A2
· 2008
[cited by applicant]
WO 2008111981A1
· 2008
[cited by applicant]
WO 2009105499A1
· 2009
[cited by applicant]
WO 2010078194A1
· 2010
[cited by applicant]
WO 2010096323A1
· 2010
[cited by applicant]
WO 2010111388A2
· 2010
[cited by applicant]
WO 2010123908A1
· 2010
[cited by applicant]
WO 2010132756A2
· 2010
[cited by applicant]
WO 2011014741A1
· 2011
[cited by applicant]
WO 2011057184A1
· 2011
[cited by applicant]
WO 2011082415A2
· 2011
[cited by applicant]
WO 2012145662A1
· 2012
[cited by applicant]
WO 2012151391A2
· 2012
[cited by applicant]
WO 2012166913A1
· 2012
[cited by applicant]
WO 2013019290A2
· 2013
[cited by applicant]
WO 2013086428A1
· 2013
[cited by applicant]
WO 2013123030A2
· 2013
[cited by applicant]
WO 2013145870A1
· 2013
[cited by applicant]
WO 2014029791A1
· 2014
[cited by applicant]
WO 2014049022A1
· 2014
[cited by applicant]
WO 2015134053A1
· 2015
[cited by applicant]
WO 2017031354A2
· 2017
[cited by applicant]
WO 2017201612A1
· 2017
[cited by applicant]
WO 2017214310A1
· 2017
[cited by applicant]
WO 2017218789A1
· 2017
[cited by applicant]
WO 2018022991A1
· 2018
[cited by applicant]
WO 2018031903A1
· 2018
[cited by applicant]
WO 2018035340A1
· 2018
[cited by applicant]
WO 2018145005A1
· 2018
[cited by applicant]
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· 2019
[cited by applicant]
WO 2019090126A1
· 2019
[cited by applicant]
WO 2020140035A1
· 2020
[cited by applicant]
Koba et al., Postepy High Med Dosw, 2005, 59, 290-8-English Abstract.
[cited by examiner]
George et al., RNA Complete BCT™: A novel blood collection tube targeting circulating RNA and extracellular vesicles, Presented at Tricon 2020 (Mar. 3, 2020) and ePoster at AACR 2020 (Apr. 27-28, 2020 & Jun. 22-24, 2020…
[cited by applicant]
Seong et al., Stability of Draw Time microRNA Concentration in RNA Complete BCT™, AACR 2020 (Apr. 27-28, 2020 & Jun. 22-24, 2020).
[cited by applicant]
Liberti et al., Bioreceptor Fenofluids: Novel Characteristics and their Utility in Medical Applications, Supplied by the British Library, Kluwer Academic Publishers; (1996).
[cited by applicant]
Liu et al., Placental mosaicism for Trisomy 13: a challenge in providing the cell-free fetal DNA testing, Journal of assisted reproduction and genetics, 31(5):589-94 (2014).
[cited by applicant]
Lo et al., Commentary: fetal-derived paternally inherited genetic markers in maternal plasma, from molecular testing in laboratory medicine, AACC Press, 264-265 (2002).
[cited by applicant]
Lo et al., Increased Fetal DNA Concentrations in the Plasma of Pregnant Women Carrying Fetuses with Trisomy 21, Clinical Chemistry, 45(10):1747-1751 (1999).
[cited by applicant]
Lo et al., Noninvasive prenatal diagnosis for fetal chromosomal aneuploidies by maternal plasma nucleic acid analysis, Clinical Chemistry, American Association for Clinical Chemistry, 54(3):461-466 (2008).
[cited by applicant]
Lo et al., Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma, N engl. J. med., 1734-1738 (1998).
[cited by applicant]
Lo et al., Prenatal diagnosis: progress through plasma nucleic acids, nature reviews genetics 8:71-77 (2007).
[cited by applicant]
Lo et al., Presence of Fetal DNA in Maternal Plasma and Serum, The Lancet, 350:485-87 (1997).
[cited by applicant]
Lo et al., Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis, Am. J. Hum. Genet., 62:768-775 (1998).
[cited by applicant]
Lo et al., Quantitative Analysis of Fetal DNA in Maternal Plasma and Serum: Implications for Noninvasive Prenatal Diagnosis, by The American Society of Human Genetics, 62:768-775 (1998).
[cited by applicant]
Lo et al., Rapid clearance of fetal DNA from maternal plasma, Am. J. Hum. Genet., 64:218-224 (1999).
[cited by applicant]
Lo, Circulating Nucleic Acids in Plasma and Serum: An Overview, Annals of the New York Academy of Sciences, 945:1-291 (2001).
[cited by applicant]
Lo, Fetal DNA in maternal plasma/serum: the first 5 years, Pediatr. Res., 53(1):16-17 (2003).
[cited by applicant]
Lo, Fetal DNA in Maternal Plasma: Biology and Diagnostic Applications, Clinical Chemistry, 46(12):1903-1906 (2000).
[cited by applicant]
Lo, Fetal Nucleic Acids in Maternal Plasma, Toward the Development of Noninvasive Prenatal Diagnosis of Fetal Chromosomal Aneuploidies, New York Academy of Sciences, 1137:140-143 (2008).
[cited by applicant]
Lo, Introduction: Plasma DNA and Urinary DNA, pp. 261-263, from Bruns et al. (eds.), Molecular Testing in Laboratory Medicine: Selections from Clinical Chemistry, 1998-2001, AACC Press (2002).
[cited by applicant]
Lo, Molecular Testing of Urine: Catching DNA on the way out, Clinical Chemistry, 46(8):1039-40 (2000).
[cited by applicant]
Locke et al., DNA Methylation Cancer Biomarkers: Translation to the Clinic, Frontiers in Genetics, 10(1150):1-22 (2019).
[cited by applicant]
Loftsson et al., Cyclodextrins in drug delivery, Expert Opin. Drug Deliv., 2:335-351 (2005).
[cited by applicant]
Loftsson et al., Self-association of cyclodextrins and cyclodextrin complexes, J. Pharm. Sci., 93(5):1091-1099 (2004).
[cited by applicant]
Lu et al., Detection and Characterization of Circulating Tumour Cells from Frozen Peripheral Blood Mononuclear Cells, Journal of Circulating Biomarkers, 35(12):1243-6 (2015).
[cited by applicant]
Lui et al., Circulating DNA in plasma and serum: Biology, Preanalytical issues and diagnostic applications, Clin. Chem. Lab. Med., 40(10):962-968 (2002).
[cited by applicant]
Lui et al., Predominant hematopoietic origin of cell-free DNA in plasma and serum after sex-mismatched bone marrow transplantation, Clinical Chemistry, 48(3):421-427 (2002).
[cited by applicant]
Luk et al., CTC-mRNA (AR-V7) Analysis from Blood Samples—Impact of Blood Collection Tube and Storage Time, Int. J. Mol. Sci., 18(5):1047 (2017).
[cited by applicant]
Lutz et al., Release of spectrin-free vesicles from human erythrocytes during ATP depletion. I. Characterization of spectrin-free vesicles, J. Cell Biol., 73(3):548-60 (1977).
[cited by applicant]
Machaca et al., Characterization of apoptosis-like endonuclease activity in avian thymocytes, Biology of the Cell, 76(1):15-22 (1992).
[cited by applicant]
Madabusi et al., RNA extraction for arrays, Methods in Enzymology, 411:1-14 (2006).
[cited by applicant]
Magnette et al., Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories, Thromb J., 14:49 (2016).
[cited by applicant]
Mahammad et al., Cholesterol depletion using methyl-beta-cyclodextrin, Methods in Membrane Lipids, 91-102 (2015).
[cited by applicant]
Makhro et al., Red cell properties after different modes of blood transportation, Front Physiol., 7:288 (2016).
[cited by applicant]
Markus et al., Evaluation of pre-analytical factors affecting plasma DNA analysis, Sci. Rep., 8(1):7375 (2017).
[cited by applicant]
Marrinucci et al., Cytomorphology of circulating colorectal tumor cells:a small case series, J. Oncol., 2010:861341 (2010).
[cited by applicant]
Maunier et al., Can stabilization of whole blood samples with Cytochex (Trademark) allow test batching of CD55 and CD59 deficiency flow cytometry analysis?, Cellquant-redquant CD55/CD59, 1-8 (2012).
[cited by applicant]
May et al., How Many Species Are There On Earth?, Science, 241:1441-1449 (1988).
[cited by applicant]
McCoy, Ch. 10: Preparation of cells from blood, Methods in Cell Biology, 63 (2001).
[cited by applicant]
McCullough et al., Non-invasive prenatal chromosomal aneuploidy testing-clinical experience: 100,000 clinical samples, PLoS One, 9(10):e109173 (2014).
[cited by applicant]
Mellert et al., Development and clinical utility of a blood-based test service for the rapid identification of actionable mutations in non-small cell lung carcinoma, J. Mol. Diag., 19(3):404-416 (2017).
[cited by applicant]
Merriam-Webster'S Medical Dictionary, p. 606, Springfield, MA: Merriam-Webster Incorporated (1995).
[cited by applicant]
Milde et al., Improved DNA typing of human urine by adding EDTA, Int. J. Legal Med., 112(3):209-210 (1999).
[cited by applicant]
Miller et al., A Simple Salting Out Procedure for Extracting DNA from Human Nucleated Cells, Nucleic Acids Research, 16(3):1215 (1988).
[cited by applicant]
Miller-Lindholm et al., Streck cell preservative preserves bone marrow specimens, Streck Cell Preservative Application Note Issue 1, 320024-2, 1-4 (2004).
[cited by applicant]
Minear et al., Global perspectives on clinical adoption of NIPT, Prenat. Diagn., 35(10):959-967 (2015).
[cited by applicant]
Modrek et al., Genome-wide Detection of Alternative Splicing in Expressed Sequences of Human Genes, Nucleic Acid Research, 29(13):2850-2859 (2001).
[cited by applicant]
Motoyama et al., Effect of 2,6-di-O-methyl-alpha-cyclodextrin on hemolysis and morphological change in rabbit's red blood cells, Eur. J. Pharm. Sci., 29(2):111-9 (2006).
[cited by applicant]
Motoyama et al., Involvement of lipid rafts of rabbit red blood cells in morphological changes induced by methylated beta-cyclodextrins, Biol. Pharm. Bull., 32(4):700-5 (2009).
[cited by applicant]
Murray et al., “Future-Proofing” Blood Processing for Measurement of Circulating miRNAs in Samples from Biobanks and Prospective Clinical Trials, Cancer Epidemiol Biomarkers Prev., 27(2):208-218 (2018).
[cited by applicant]
Murugesan et al., Investigation of Preanalytical Variables Impacting Pathogen Cell-Free DNA in Blood and Urine, Journal of Clinical Microbiology, 57(11):1-13 (2019).
[cited by applicant]
Nace et al., Evaluation of Streck tissue fixative, a nonformalin fixative for preservation of stool samples and subsequent parasitologic examination, J. Clin. Microbiology, 37(12):4113-4119 (1999).
[cited by applicant]
Nair et al., An observational study of circulating tumor cells and (18) F-FDG PET uptake in patients with treatment-naive non-small cell lung cancer, PloS One, 8(7):e67733 (2013).
[cited by applicant]
Nicholson et al., Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry, J. Immunol. Methods, 160:215-218 (1993).
[cited by applicant]
Chan et al., Hypermethylated RASSFIA in maternal Plasma: A Universal Fetal DNA Marker that Improves the Reliability of Noninvasive Prenatal Diagnosis, Clinical Chemistry, 52(12):2211-2218 (2006).
[cited by applicant]
Chan et al., Size distributions of maternal and fetal DNA in maternal plasma, clinical chemistry, 50(1):88-92 (2004).
[cited by applicant]
Chang et al., Review of the clinical applications and technological advances of circulating tumor DNA in cancer monitoring, The Clin. Risk Manag., 13:1363-1374 (2017).
[cited by applicant]
Cherepanova et al., Immunochemical assay for deoxyribonuclease activity in body fluids, Journal of immunological methods, 325(1):96-103 (2007).
[cited by applicant]
Chinnapapagari et al., Treatment of maternal blood samples with formaldehyde does not alter the proportion of circulatory fetal nucleic acids (DNA and mRNA) in maternal plasma, Clin Chem., 51(3):652-5 (2005).
[cited by applicant]
Chiu et al., Effects of blood-processing protocols on fetal and total DNA quantification in maternal plasma, Clin. Chem., 47(9):1607-1613 (2001).
[cited by applicant]
Chiu et al., Effects of Blood-Processing Protocols on Fetal and Total DNA Quantification in Maternal Plasma, Clinical Chemistry, 47(9):1607-1613 (2001).
[cited by applicant]
Chu et al., ESR1 mutations in circulating plasma tumor DNA from metastatic breast cancer patients, Clin. Cancer Res., 22(4):993-999 (2016).
[cited by applicant]
Chudziak et al., Clinical evaluation of a novel microfluidic device for epitope-independent enrichment of circulating tumour cells in patients with small cell lung cancer, The Analyst, 141(2):669-78 (2015).
[cited by applicant]
Chung et al., Detrimental Effect of Formaldehyde on Plasma RNA Detection, Clin. Chem., 51(6):1074-6 (2005).
[cited by applicant]
Chung et al., Lack of Dramatic Enrichment of Fetal DNA in Maternal Plasma by Formaldehyde Treatment, Clinical Chemistry, 51(3):655-8 (2005).
[cited by applicant]
Chutkan et al., Quantitative and qualitative preparations of bacterial outer membrane vesicles, Methods Mol. Biol., 966:259-272 (2013).
[cited by applicant]
Clark-Ganheart et al., Use of Cell-Free DNA in the Investigation of Intrauterine Fetal Demise and Miscarriage, Obstetrics & Gynecology, 125(6):1321-9 (2015).
[cited by applicant]