Rabies virus vaccine
The present invention provides a vaccine for rabies virus and methods of making and using the vaccine alone, or in combinations with other protective agents.
1. A vaccine to aid in the prevention of disease due to rabies virus comprising a Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle (RP), and comprising a nucleotide sequence encoding a rabies virus antigen and a pharmaceutically acceptable carrier; wherein the nucleotide sequence encoding the rabies virus antigen comprises at least 85% sequence identity to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 4.
2. The vaccine of claim 1 , that comprises one or more additional alphavirus RNA replicon particles which encodes a second rabies virus antigen that originates from a different strain of rabies virus than the one that said rabies virus antigen originates from.
3. The vaccine of claim 2 , wherein the second rabies virus antigen is a glycoprotein (G) or an antigenic fragment thereof.
4. The vaccine of claim 3 , wherein the one or more additional alphavirus RNA replicon particles are VEE alphavirus RNA replicon particles.
5. The vaccine of claim 1 , wherein the rabies virus antigen is a rabies virus glycoprotein (G) having an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 2.
6. The vaccine of claim 5 , wherein an antibody is induced in a mammal when said mammal is immunized with the vaccine.
7. The vaccine of claim 6 , wherein the mammal is selected from the group consisting of a canine, a feline, an equine, a ferret, a sheep, and a bovine.
8. The vaccine of claim 1 , that further comprises at least one non-rabies virus antigen for eliciting protective immunity to a non-rabies virus pathogen.
9. The vaccine of claim 1 , that further comprises at least one recombinant vector that comprises a nucleotide sequence encoding at least one protein antigen or antigenic fragment thereof that originates from a non-rabies virus pathogen.
10. The vaccine of claim 1 , that further comprises an alphavirus RNA replicon particle comprising a nucleotide sequence encoding at least one protein antigen or antigenic fragment thereof that originates from a non-rabies virus pathogen.
11. The vaccine of claim 1 , that is a non-adjuvanted vaccine.
12. A method of immunizing a mammal against rabies virus comprising administering to the mammal an immunologically effective amount of the vaccine of claim 1 .
13. The method of claim 12 , wherein the mammal is selected from the group of a canine, a feline, and an equine.
14. The vaccine of claim 1 , that comprises about 1×10 6 to about 1×10 9 RPs.
15. The vaccine of claim 11 , that is a one dose vaccine.
16. The vaccine of claim 1 , wherein the rabies virus antigen is a rabies virus glycoprotein (G) having an amino acid sequence of SEQ ID NO: 2.
17. The vaccine of claim 1 , wherein the nucleotide sequence encoding the rabies virus antigen comprises the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 4.
18. The vaccine of claim 1 , wherein the prevention of disease due to rabies virus in canines or felines comprises at least a three-year duration of immunity to said disease.