Hemipteran active insecticidal protein
This disclosure concerns compositions and methods for novel pesticidal proteins, polynucleotides encoding such proteins, use of such novel pesticidal proteins to control Hemipteran/Lepidopteran plant pests, and transgenic plants that produce, and are protected, by these novel pesticidal proteins are described.
1. A DNA construct comprising one or more heterologous regulatory sequences directing expression of a recombinant nucleic acid molecule encoding a polypeptide that has at least 98% sequence identity with SEQ ID NO:2, wherein the polypeptide has insecticidal activity.
2. The DNA construct of claim 1 , wherein the polypeptide further comprises a signal sequence or a leader sequence.
3. The DNA construct of claim 1 , further comprising a nucleic acid sequence encoding an additional transgenic trait.
4. The DNA construct of claim 3 , wherein the one or more additional transgenic trait is selected from the group consisting of insect resistance, herbicide tolerance, nitrogen use efficiency, small RNA expression, site specific nuclease, water use efficiency, and nutritional quality.
5. An isolated polypeptide encoded by the DNA construct of claim 2 .
6. A composition comprising an insecticidally-effective amount of the polypeptide of claim 5 .
7. The composition of claim 6 , further comprising one or more pesticidal proteins selected from the group consisting of a Cry1 protein, a Cry2 protein, a Cry3 protein, a Cry4 protein, a CryS5 protein, a Cry6 protein, a Cry7 protein, a Cry8 protein, a Cry9 protein, a Cry15 protein, Cry22 protein, a Cry23 protein, a Cry32 protein, a Cry34 protein, a Cry35 protein, a Cry36 protein, a Cry37 protein, a Cry43 protein, a Cry46 protein, a Cry51 protein, a Cry55 protein, a Cry binary toxin, a Cyt protein, a VIP toxin, a SIP protein, an insecticidal lipase, an insecticidal chitinase, and a snake venom protein.
8. The composition of claim 6 , further comprising one or more pesticidal small RNA molecules.
9. The DNA construct of claim 1 , wherein the polypeptide is orally active.
10. The DNA construct of claim 1 , wherein the polypeptide has insecticidal activity against an insect pest in the order Hemiptera.
11. The DNA construct of claim 1 , wherein the polypeptide has insecticidal activity against an insect pest in the family Pentatomidae.
12. The DNA construct of claim 1 , wherein the polypeptide comprises any one or more amino acid modification relative to position 6 of SEQ ID NO:2, position 13 of SEQ ID NO:2, position 18 of SEQ ID NO:2, position 23 of SEQ ID NO:2, position 28 of SEQ ID NO:2, and/or position 75 of SEQ ID NO:2.
13. A method for controlling an insect pest population, the method comprising contacting the insect pest population with an insecticidally-effective amount of the polypeptide of claim 5 .
14. A method of inhibiting growth or killing an insect pest, the method comprising contacting the insect pest with an insecticidally-effective amount of the polypeptide of claim 5 .
15. A method for controlling an insect pest population resistant to a pesticidal protein, the method comprising contacting the insect pest population with an insecticidally-effective amount of the recombinant polypeptide of claim 5 .
16. The DNA construct of claim 1 , wherein the protein comprises the protein motif sequence of SEQ ID NO:37.