Plant promoter for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5′ UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a promoter operably linked to a heterologous polynucleotide sequence; wherein said promoter comprises a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:2, wherein SEQ ID NO:2 drives above ground green tissue expression of the heterologous polynucleotide sequence.
2. The nucleic acid vector of claim 1 , wherein said promoter is 821 bp in length.
3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:2.
4. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.
5. The nucleic acid vector of claim 4 , wherein the heterologous coding sequence encodes a selectable marker protein, an insecticidal resistance protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a small RNA molecule, a nutritional quality protein, or a DNA binding protein.
6. The nucleic acid vector of claim 1 , further comprising a terminator polynucleotide sequence.
7. The nucleic acid vector of claim 1 , further comprising a 3′ untranslated polynucleotide sequence.
8. The nucleic acid vector of claim 1 , further comprising a 5′ untranslated polynucleotide sequence.
9. The nucleic acid vector of claim 1 , further comprising an intron sequence.
10. The nucleic acid vector of claim 1 , wherein said promoter has tissue preferred expression.
11. A transgenic plant comprising a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:2 operably linked to a heterologous coding sequence.
12. The transgenic plant of claim 11 , wherein said plant is selected from the group consisting of Zea mays , wheat, rice, sorghum, oats, rye, bananas, sugar cane, Glycine max , cotton, Arabidopsis , tobacco, sunflower, and canola.
13. The transgenic plant of claim 12 , wherein said plant is Glycine max.
14. The transgenic plant of claim 13 , wherein the heterologous coding sequence is inserted into the genome of said plant.
15. The transgenic plant of claim 11 , wherein the heterologous coding sequence further comprises a 3′ untranslated sequence.
16. A method for producing a transgenic plant cell, the method comprising the steps of:
a) transforming a plant cell with a gene expression cassette comprising a GmPSID2 promoter, wherein the GmPSID2 promoter shares polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:2, operably linked to at least one polynucleotide sequence of interest;
b) isolating the transformed plant cell comprising the gene expression cassette; and,
c) producing a transgenic plant cell comprising the GmPSID2 promoter operably linked to at least one polynucleotide sequence of interest, wherein the GmPSID2 promoter drives above ground green tissue expression of the polynucleotide sequence of interest.
17. A method for expressing a polynucleotide sequence of interest in a plant cell, the method comprising introducing into the plant cell a polynucleotide sequence of interest operably linked to a GmPSID2 promoter, wherein the GmPSID2 promoter shares at least 98% sequence identity with SEQ ID NO:2, wherein the GmPSID2 promoter drives above ground green tissue expression of the polynucleotide sequence.