COMPOSITIONS OF ENGINEERED EXOSOMES AND METHODS OF LOADING LUMINAL EXOSOMES PAY-LOADS
The present invention relates to methods of preparing a therapeutic exosome using proteins newly identified to be enriched in the lumen of exosomes. Specifically, the present invention provides methods of localizing a therapeutic peptide or protein in exosomes. The methods involve generation of lumen-engineered exosomes that include one or more of the exosome proteins at higher concentrations, a modification or a fragment of the exosome protein, or a fusion protein of the exosome protein and a therapeutic or cargo protein.
1 . An exosome comprising a target protein, wherein at least a part of the target protein is expressed from an exogenous sequence, and the target protein comprises MARCKS, MARCKSL1, BASP1 or a fragment or a modification thereof.
2 . The exosome of claim 1 , wherein the target protein is present in the exosome at a higher density than a different target protein in a different exosome, wherein the different target protein comprises a conventional exosome protein or a variant thereof.
3 . The exosome of claim 2 , wherein the conventional exosome protein is selected from the group consisting of CD9, CD63, CD81, PDGFR, GPI anchor proteins, lactadherin, LAMP2, LAMP2B, and a fragment thereof.
4 . The exosome of any of claims 1 - 3 , wherein the exosome is produced from a cell genetically modified to comprise the exogenous sequence, optionally wherein the cell is an HEK293 cell.
5 . The exosome of claim 4 , wherein the cell comprises a plasmid comprising the exogenous sequence.
6 . The exosome of claim 4 , wherein the cell comprises the exogenous sequence inserted into a genome of the cell.
7 . The exosome of claim 6 , wherein the exogenous sequence is inserted into a genomic site located 3′ or 5′ relative to a genomic sequence encoding MARCKS, MARCKSL1, or BASP1.
8 . The exosome of claim 6 , wherein the exogenous sequence is inserted into a genomic sequence encoding MARCKS, MARCKSL1, or BASP1.
9 . The exosome of any of claims 1 - 8 , wherein the target protein is a fusion protein comprising MARCKS, MARCKSL1, BASP1, or a fragment thereof, and a therapeutic peptide.
10 . The exosome of claim 9 , wherein the therapeutic peptide is selected from the group consisting of a natural peptide, a recombinant peptide, a synthetic peptide, or a linker to a therapeutic compound.
11 . The exosome of claim 9 , wherein the therapeutic compound is selected from the group consisting of nucleotides, amino acids, lipids, carbohydrates, and small molecules.
12 . The exosome of claim 9 , wherein the therapeutic peptide is an antibody or a fragment or a modification thereof.
13 . The exosome of claim 9 , wherein the therapeutic peptide is an enzyme, a ligand, a receptor, a transcription factor, or a fragment or a modification thereof.
14 . The exosome of claim 9 , wherein the therapeutic peptide is an antimicrobial peptide or a fragment or a modification thereof.
15 . The exosome of any of claims 1 - 14 , further comprising a second target protein, wherein the second target protein comprises MARCKS, MARCKSL1, BASP1, or a fragment thereof.
16 . The exosome of any of claims 1 - 14 , further comprising a second target protein, wherein the second target protein comprises PTGFRN, BSG, IGSF2, IGSF3, IGSF8, ITGB1, ITGA4, SLC3A2, ATP transporter or a fragment thereof.
17 . The exosome of any of claims 1 - 15 , wherein the target protein comprises a peptide of (M)(G)(G/A/S)(K/Q)(L/F/S/Q)(S/A)(K)(K) (SEQ ID NO: 118).
18 . The exosome of any of claims 1 - 15 , wherein the target protein comprises a peptide of (M)(G)(π)(X)(Φ/π)(π)(+)(+), wherein each parenthetical position represents an amino acid, and wherein π is any amino acid selected from the group consisting of (Pro, Gly, Ala, Ser), X is any amino acid, Φ is any amino acid selected from the group consisting of (Val, Ile, Leu, Phe, Trp, Tyr, Met), and (+) is any amino acid selected from the group consisting of (Lys, Arg, His); and wherein position five is not (+) and position six is neither (+) nor (Asp or Glu).
19 . The exosome of any of claims 1 - 15 , wherein the target protein comprises a peptide of (M)(G)(π)(ξ)(Φ/π)(S/A/G/N)(+)(+), wherein each parenthetical position represents an amino acid, and wherein π is any amino acid selected from the group consisting of (Pro, Gly, Ala, Ser), ξ is any amino acid selected from the group consisting of (Asn, Gln, Ser, Thr, Asp, Glu, Lys, His, Arg), Φ is any amino acid selected from the group consisting of (Val, Ile, Leu, Phe, Trp, Tyr, Met), and (+) is any amino acid selected from the group consisting of (Lys, Arg, His); and wherein position five is not (+) and position six is neither (+) nor (Asp or Glu).
20 . The exosome of any of claims 17 - 19 , wherein the target protein comprises a peptide of any one of SEQ ID NO: 4-110.
21 . The exosome of any of claims 17 - 19 , wherein the target protein comprises a peptide of MGXKLSKKK, wherein X is any amino acid (SEQ ID NO: 116).
22 . The exosome of claim 21 , wherein the target protein comprises a peptide of SEQ ID NO: 110.
23 . The exosome of claim 20 , wherein the target protein comprises the peptide of SEQ ID NO: 13.
24 . The exosome of any of claims 1 - 23 , wherein the target protein further comprises a cargo peptide.
25 . A pharmaceutical composition comprising the exosome of any of claims 1 - 24 and an excipient.
26 . The pharmaceutical composition of claim 25 , substantially free of macromolecules, wherein the macromolecules are selected from nucleic acids, exogenous proteins, lipids, carbohydrates, metabolites, and a combination thereof.
27 . A population of cells for producing the exosome of any of claims 1 - 24 .
28 . The population of cells of claim 27 , comprising an exogenous sequence encoding the target protein comprising MARCKS, MARCKSL1, BASP1 or a fragment or a modification thereof.
29 . The population of cells of claim 28 , further comprising a second exogenous sequence encoding a second target protein, wherein the second target protein comprises MARCKS, MARCKSL1, BASP1 or a fragment or a modification thereof.
30 . The population of cells of claim 28 , further comprising a second exogenous sequence encoding a second target protein, wherein the second target protein comprises PTGFRN, BSG, IGSF2, IGSF3, IGSF8, ITGB1, ITGA4, SLC3A2, ATP transporter or a fragment thereof.
31 . The population of cells of any of claims 27 - 30 , wherein the exogenous sequence is inserted into a genomic sequence encoding MARCKS, MARCKSL1, or BASP1, wherein the exogenous sequence and the genomic sequence encodes the target protein.
32 . The population of cells of any of claims 27 - 30 , wherein the exogenous sequence is in a plasmid.
33 . The population of cells of any of claims 27 - 32 , wherein the exogenous sequence encodes a therapeutic peptide.
34 . The population of cells of claim 33 , wherein the therapeutic peptide is selected from a group consisting of a natural peptide, a recombinant peptide, a synthetic peptide, or a linker to a therapeutic compound.
35 . The population of cells of claim 33 , wherein the therapeutic compound is selected from the group consisting of nucleotides, amino acids, lipids, carbohydrates, and small molecules.
36 . The population of cells of claim 33 , wherein the therapeutic peptide is an antibody or a fragment or a modification thereof.
37 . The population of cells of claim 33 , wherein the therapeutic peptide is an enzyme, a ligand, a receptor, a transcription factor, or a fragment or a modification thereof.
38 . The population of cells of claim 33 , wherein the therapeutic peptide is an antimicrobial peptide or a fragment or a modification thereof.
39 . The population of cells of claim 31 , wherein the exogenous sequence encodes a targeting moiety.
40 . The population of cells of claim 39 , wherein the targeting moiety is specific to an organ, a tissue, or a cell.
41 . The population of cells of claim 30 , wherein the second target protein further comprises a targeting moiety.
42 . The population of cells of claim 41 , wherein the targeting moiety is specific to an organ, a tissue, or a cell.
43 . A polypeptide for modifying an exosome, comprising a sequence of (i) (M)(G)(G/A/S)(K/Q)(L/F/S/Q)(S/A)(K)(K) (SEQ ID NO: 118); (ii) (M)(G)(π)(X)(Φ/π)(π)(+)(+), wherein each parenthetical position represents an amino acid, and wherein π is any amino acid selected from the group consisting of (Pro, Gly, Ala, Ser), X is any amino acid, Φ is any amino acid selected from the group consisting of (Val, Ile, Leu, Phe, Trp, Tyr, Met), and (+) is any amino acid selected from the group consisting of (Lys, Arg, His); and wherein position five is not (+) and position six is neither (+) nor (Asp or Glu); or (ii) (M)(G)(π)(ξ)(Φ/π)(S/A/G/N)(+)(+), wherein each parenthetical position represents an amino acid, and wherein π is any amino acid selected from the group consisting of (Pro, Gly, Ala, Ser), ξ is any amino acid selected from the group consisting of (Asn, Gln, Ser, Thr, Asp, Glu, Lys, His, Arg), Φ is any amino acid selected from the group consisting of (Val, Ile, Leu, Phe, Trp, Tyr, Met), and (+) is any amino acid selected from the group consisting of (Lys, Arg, His); and wherein position five is not (+) and position six is neither (+) nor (Asp or Glu).
44 . The polypeptide of claim 43 , comprising a sequence of any of SEQ ID NO: 4-110.
45 . The polypeptide of claim 43 , comprising a sequence of SEQ ID NO: 13.
46 . The polypeptide of claim 43 , comprising a sequence of SEQ ID NO: 110.
47 . The polypeptide of claim 43 , comprising a sequence of MGXKLSKKK, wherein X is any amino acid (SEQ ID NO: 116).
48 . The polypeptide of any of claims 43 - 47 , wherein the polypeptide is fused to a cargo peptide.
49 . The polypeptide of claim 48 , wherein the polypeptide is fused to the N-terminus of the cargo peptide.
50 . A polynucleotide construct comprising a coding sequence encoding the polypeptide of any of claims 43 - 49 .
51 . The polynucleotide construct of claim 50 , wherein the coding sequence is codon optimized.
52 . A method of making an engineered exosome, comprising the steps of:
a. introducing into a cell a nucleic acid construct encoding a fusion polypeptide comprising (i) a first sequence encoding MARCKS, MARCKSL1, BASP1 or a fragment or a modification thereof, and (ii) a second sequence encoding a cargo peptide;
b. maintaining the cell under conditions allowing the cell to express the fusion polypeptide; and
c. obtaining the engineered exosome comprising the fusion polypeptide from said cell.
53 . The method of claim 52 , wherein the first sequence comprises a sequence of (i) (M)(G)(G/A/S)(K/Q)(L/F/S/Q)(S/A)(K)(K) (SEQ ID NO: 118); (ii) (M)(G)(π)(X)(Φ/π)(π)(+)(+), wherein each parenthetical position represents an amino acid, and wherein π is any amino acid selected from the group consisting of (Pro, Gly, Ala, Ser), X is any amino acid, Φ is any amino acid selected from the group consisting of (Val, Ile, Leu, Phe, Trp, Tyr, Met), and (+) is any amino acid selected from the group consisting of (Lys, Arg, His); and wherein position five is not (+) and position six is neither (+) nor (Asp or Glu); or (ii) (M)(G)(π)(ξ)(Φ/π)(S/A/G/N)(+)(+), wherein each parenthetical position represents an amino acid, and wherein π is any amino acid selected from the group consisting of (Pro, Gly, Ala, Ser), ξ is any amino acid selected from the group consisting of (Asn, Gln, Ser, Thr, Asp, Glu, Lys, His, Arg), Φ is any amino acid selected from the group consisting of (Val, Ile, Leu, Phe, Trp, Tyr, Met), and (+) is any amino acid selected from the group consisting of (Lys, Arg, His); and wherein position five is not (+) and position six is neither (+) nor (Asp or Glu).
54 . The method of any of claims 52 - 53 , wherein the first sequence comprises any of SEQ ID NO: 4-110.
55 . The method of claim 54 , wherein the first sequence comprises SEQ ID NO: 13.
56 . The method of claim 54 , wherein the first sequence comprises SEQ ID NO: 110.
57 . The method of claim 53 , wherein the first sequence comprises MGXKLSKKK, wherein X is any amino acid (SEQ ID NO: 116).
58 . The method of any of claims 52 - 57 , wherein the fusion polypeptide is present in the lumen of the engineered exosome at a higher density than a different target protein in a different exosome, wherein the different target protein comprises a conventional exosome protein or a variant thereof.
59 . The method of claim 58 , wherein the fusion polypeptide is present at more than 2 fold higher density than the different target protein in the different exosome.
60 . The method of claim 59 , wherein the fusion polypeptide is present at more than 4 fold, 16 fold, 100 fold, or 10,000 fold higher density than the different target protein in the different exosome.