Vaccine composition for classical swine fever and preparation method thereof
Provided is a swine fever antigen fused with a porcine Fc fragment, and more particularly, to a vaccine composition having an autoimmune-enhancing effect by binding the Fc fragment to a swine fever antigen, and a preparation method thereof.
1. A method for treating classical swine fever, comprising administering to a subject in need thereof a composition comprising a classical swine fever antigen E2 protein fused with a porcine Fc fragment consisting of the amino acid sequence of SEQ ID No. 4 as an active ingredient.
2. The method for treating classical swine fever of claim 1 , wherein the E2 protein comprises the an amino acid sequence of SEQ ID No. 18.
3. The method for treating classical swine fever of claim 1 , wherein the E2 protein is fused with the Fc fragment to have self-adjuvanting effect and increased solubility.
4. A recombinant vector for producing a classical swine fever antigen E2 protein fused with an Fc fragment, comprising a polynucleotide encoding a porcine Fc fragment consisting of the amino acid sequence of SEQ ID No. 4 and a polynucleotide encoding a classical swine fever antigen E2 protein.
5. The recombinant vector of claim 4 , wherein the E2 protein comprises the amino acid sequence of SEQ ID No. 18.
6. The recombinant vector of claim 4 , wherein the recombinant vector comprises a promoter gene operably and sequentially linked to the polynucleotide encoding a classical swine fever antigen E2 protein and the polynucleotide encoding a porcine Fc fragment.
7. The recombinant vector of claim 6 , wherein the promoter is one or more selected from the group consisting of a cauliflower mosaic virus-derived 35S promoter, a cauliflower mosaic virus-derived 19S RNA promoter, an actin protein promoter of a plant, a ubiquitin protein promoter, a cytomegalovirus (CMV) promoter, a simian virus 40 (SV40) promoter, a respiratory syncytial virus (RSV) promoter, a pEMU promoter, an MAS promoter, a histone promoter, a Clp promoter, and an elongation factor-1 alpha (EF-1α) promoter.
8. The recombinant vector of claim 4 , wherein the recombinant vector further comprises a polynucleotide encoding a chaperone binding protein (BiP).
9. The recombinant vector of claim 4 , wherein the recombinant vector further comprises a gene encoding SEQ ID NO: 20.
10. The recombinant vector of claim 4 , wherein the recombinant vector further comprises a 5′ untranslated region (UTR) site gene of M17.
11. The recombinant vector of claim 4 , wherein the recombinant vector increases an expression level of the classical swine fever antigen E2 protein fused with the Fc fragment.
12. A transgenic organism transformed with the recombinant vector of claim 4 .
13. A method for producing a classical swine fever antigen E2 protein fused with an Fc fragment and having an autoimmune-enhancing effect, the method comprising:
(a) culturing a transgenic organism transformed with the recombinant vector of claim 4 ; and
(b) isolating a classical swine fever E2 protein fused with an Fc fragment from the transgenic organism or culture solution and purifying the classical swine fever E2 protein fused with the Fc fragment.
14. The recombinant vector of claim 4 , wherein the E2 protein is fused with the Fc fragment to have self-adjuvanting effect and increased solubility.