Water-reconstitutable culture medium resistant to liquefaction by microorganisms
A device for growing microorganisms is provided. The device includes a body member comprising a self-supporting, waterproof substrate, a layer of adhesive disposed on the substrate, a powder adhered to the adhesive, a cover sheet adhered to the body member, and a dry composition including a mixture of hydroxypropylmethylcellulose and guar gum adhered to the cover sheet or the substrate. The powder comprises a cold-water-soluble gelling agent and one or more nutrient for growing microorganisms. The composition, when reconstituted with water, resists liquefaction by microorganisms A method of culturing microorganisms using the device is also provided.
1. A device for growing microorganisms, comprising:
i. a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces, a layer of adhesive, and a cold-water-soluble powder;
wherein said layer of adhesive is disposed on said upper surface of said substrate;
wherein said adhesive is non-inhibitory to the growth of microorganisms;
wherein said cold-water-soluble powder comprises guar gum and hydroxypropylmethylcellulose and one or more nutrients for growing microorganisms;
wherein said cold-water-soluble powder is adhered to said adhesive, where said hydroxypropylmethylcellulose and said guar gum are present in said cold-water-soluble powder at a mass ratio in the range of one part of said hydroxypropylmethylcellulose to one part of said guar gum to three parts of said hydroxypropylmethylcellulose to one part of said guar gum; and
ii. a cover sheet having an inner-facing surface and an outer-facing surface, comprising a dry composition and a layer of adhesive;
wherein said adhesive is adhered to the inner-facing surface of said cover sheet;
wherein said dry composition comprises a mixture of hydroxypropylmethylcellulose and guar gum;
wherein said dry composition is adhered to said inner-facing surface of said cover sheet;
wherein said device includes a microbial growth zone between said substrate and said cover sheet;
wherein said microbial growth zone is free of matrices that prevent visualization of bacterial colonies;
wherein said cover sheet is adhered to said body member so that the upper surface of said substrate faces said inner-facing surface of said cover sheet; and
wherein said hydroxypropylmethylcellulose and said guar gum are present in said dry composition at a mass ratio in the range of one part of said hydroxypropylmethylcellulose to one part of said guar gum to three parts of said hydroxypropylmethylcellulose to one part of said guar gum.
2. The device of claim 1 , the cold-water-soluble powder consists essentially of guar gum and hydroxypropylmethylcellulose.
3. The device of claim 1 , wherein the dry composition consists essentially of hydroxypropylmethylcellulose and guar gum.
4. A device for growing microorganisms, comprising:
i. a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces, and a cold-water-reconstitutable coating;
wherein said cold-water-reconstitutable coating comprises guar gum and hydroxypropylmethylcellulose and one or more nutrients for growing microorganisms;
wherein said hydroxypropylmethylcellulose and said guar gum are present in said cold-water-reconstitutable coating at a mass ratio in the range of one part of said hydroxypropylmethylcellulose to one part of said guar gum to three parts of said hydroxypropylmethylcellulose to one part of said guar gum;
wherein said cold-water-reconstitutable coating is adhered to said substrate; and
ii. a cover sheet having an inner-facing surface and an outer-facing surface, comprising a dry composition and a layer of adhesive;
wherein said adhesive is adhered to the inner-facing surface of said cover sheet;
wherein said dry composition comprises a mixture of hydroxypropylmethylcellulose and guar gum; where said hydroxypropylmethylcellulose and said guar gum are present in said dry composition at a mass ratio in the range of one part of said hydroxypropylmethylcellulose to one part of said guar gum to three parts of said hydroxypropylmethylcellulose to one part of said guar gum
wherein said dry composition is adhered to said inner-facing surface of said cover sheet;
wherein said device includes a microbial growth zone between said substrate and said cover sheet;
wherein said microbial growth zone is free of matrices that prevent visualization of bacterial colonies;
wherein said cover sheet is adhered to said body member so that the upper surface of said substrate faces said inner-facing surface of said cover sheet.
5. The device of claim 4 , wherein the cold-water-reconstitutable coating consists essentially of guar gum and hydroxypropylmethylcellulose.
6. The device of any one of claim 4 , wherein the dry composition consists essentially of hydroxypropylmethylcellulose and guar gum.
7. The device of claim 4 , further comprising a layer of adhesive disposed on the upper surface of the substrate, the adhesive being non-inhibitory to the growth of microorganisms; wherein the cold-water-reconstitutable coating is adhered to the adhesive layer.
8. The device of claim 1 , wherein the cold-water-soluble powder can comprise powdered nutrients and powdered cold-water-soluble gelling agent(s) that are present in a ratio of about 3 parts nutrients: 1 part cold-water-soluble gelling agent(s).