Compositions and methods for treating and preventing tissue injury and disease
The present invention provides novel compositions comprising multipotent cells or microvascular tissue, wherein the cells or tissue has been sterilized and/or treated to inactivated viruses, and related methods of using these compositions to treat or prevent tissue injury or disease in an allogeneic subject.
1. A method of treating an injury in a subject in need thereof, comprising administering a therapeutic agent to a subject in need thereof, wherein the therapeutic agent comprises enriched, sterilized multipotent cells, wherein the multipotent cells are sterilized by irradiation.
2. The method of claim 1 , wherein the injury is a soft-tissue injury.
3. The method of claim 2 , wherein the soft-tissue injury is ischemic.
4. The method of claim 2 , wherein the soft-tissue injury is tendinopathy.
5. The method of claim 2 , wherein the soft-tissue injury is in a ligament.
6. The method of claim 2 , wherein the soft-tissue injury is in muscle, synovium, or adipose tissue.
7. The method of claim 1 , wherein the injury is a bone defect or bone void.
8. The method of claim 1 , wherein the multipotent cells have been isolated from microvascular tissue.
9. The method of claim 1 , wherein the multipotent cells have been enriched by removal of extraneous tissue.
10. The method of claim 1 , wherein the multipotent cells have been enriched by culturing the multipotent cells.
11. The method of claim 1 , wherein administering the therapeutic agent comprises injection or surgical application to the region of injury.
12. The method of claim 1 , wherein administering the therapeutic agent comprises systemic injection.
13. The method of claim 2 , wherein the therapeutic agent is a powder, gel or ointment applied topically near the site of soft-tissue injury.
14. The method of claim 2 , wherein the soft-tissue injury is a wound.
15. The method of claim 14 , wherein the wound is selected from the group consisting of a surgical wound, a burn, a radiation burn, a diabetic wound, a pressure ulcer, a venous ulcer and a diabetic ulcer.
16. A method of treating an injury in a subject in need thereof, comprising administering a therapeutic agent to a subject in need thereof, wherein the therapeutic agent comprises enriched, sterilized multipotent cells, wherein the sterilized multipotent cells are nonviable.
17. The method of claim 16 , wherein the injury is a soft-tissue injury.
18. The method of claim 17 , wherein the soft-tissue injury is ischemic.
19. The method of claim 17 , wherein the soft-tissue injury is tendinopathy.
20. The method of claim 17 , wherein the soft-tissue injury is in a ligament.
21. The method of claim 17 , wherein the soft-tissue injury is in muscle, synovium, or adipose tissue.
22. The method of claim 16 , wherein the injury is a bone defect or bone void.
23. The method of claim 16 , wherein the multipotent cells have been isolated from microvascular tissue.
24. The method of claim 16 , wherein the multipotent cells have been enriched by removal of extraneous tissue.
25. The method of claim 16 , wherein the multipotent cells have been enriched by culturing the multipotent cells.
26. The method of claim 16 , wherein administering the therapeutic agent comprises injection or surgical application to the region of injury.
27. The method of claim 16 , wherein administering the therapeutic agent comprises systemic injection.
28. The method of claim 17 , wherein the therapeutic agent is a powder, gel or ointment applied topically near the site of soft-tissue injury.
29. The method of claim 17 , wherein the soft-tissue injury is a wound.
30. The method of claim 14 , wherein the wound is selected from the group consisting of a surgical wound, a burn, a radiation burn, a diabetic wound, a pressure ulcer, a venous ulcer and a diabetic ulcer.