Riboswitch-mediated regulatory control of gene expression in thermophilic bacteria
Methods of riboswitch-mediated gene expression in thermophiles are provided herein.
1. A non-naturally occurring riboswitch selected from the group consisting of a nucleotide sequence that is greater than 90% identical to SEQ ID NO: 1 or greater than 90% identical to SEQ ID NO: 3 wherein the non-naturally occurring riboswitch is operably linked to a heterologous promoter wherein the non-naturally occurring riboswitch is capable of regulating the expression of a gene of interest in a non-naturally occurring thermophilic organism.
2. The non-naturally occurring riboswitch of claim 1 wherein the riboswitch is capable of regulating the expression of a gene of interest at temperatures greater than 50 degrees Celsius.
3. The non-naturally occurring riboswitch of claim 1 wherein the expression of the gene of interest is downregulated.
4. The non-naturally occurring riboswitch of claim 1 wherein the expression of the gene of interest is upregulated.
5. The non-naturally occurring riboswitch of claim 4 wherein the gene of interest is expressed up to 29-fold over wild-type expression.
6. The non-naturally occurring riboswitch of claim 1 wherein the gene of interest is adhE.